用胶束电动毛细管色谱法分析生物基质中的甲喹酮。与气相色谱-质谱联用的比较。
Analysis of methaqualone in biological matrices by micellar electrokinetic capillary chromatography. Comparison with gas chromatography-mass spectrometry.
作者信息
Plaut O, Girod C, Staub C
机构信息
Institute of Forensic Medicine, University of Geneva, Switzerland.
出版信息
Forensic Sci Int. 1998 Apr 5;92(2-3):219-27. doi: 10.1016/s0379-0738(98)00018-8.
The analysis of methaqualone (MTQ) in biological matrices by capillary electrophoresis (CE) is described. This methods uses liquid-liquid extraction and micellar electrokinetic capillary chromatography (MECC), an operation mode of CE. Separations are made using a 25 cm long capillary and a borate/phosphate buffer at pH 8.2. Using gas chromatography with mass spectrometry detection (GC-MS) as reference method, MTQ has been analyzed in urine, blood, gastric content and hair. For hair analysis, supercritical fluid extraction was compared with liquid-liquid extraction. Linearity was established in urine and blood between 0.25 and 10.0 micrograms/ml. MTQ recovery from blood was estimated at 60%. The limit of detection of this method in urine is about 0.10 microgram/ml. Drawbacks and advantages of MECC over GC-MS are discussed.
描述了通过毛细管电泳（CE）分析生物基质中的甲喹酮（MTQ）。该方法采用液液萃取和胶束电动毛细管色谱法（MECC），这是CE的一种操作模式。使用25 cm长的毛细管和pH 8.2的硼酸盐/磷酸盐缓冲液进行分离。以气相色谱-质谱检测（GC-MS）作为参考方法，对尿液、血液、胃内容物和毛发中的MTQ进行了分析。对于毛发分析，将超临界流体萃取与液液萃取进行了比较。在尿液和血液中，0.25至10.0微克/毫升之间建立了线性关系。血液中甲喹酮的回收率估计为60%。该方法在尿液中的检测限约为0.10微克/毫升。讨论了MECC相对于GC-MS的缺点和优点。
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