Buscemi L, Tagliabracci A, Sassaroli C, Bianchi F, Canestrari S, Rodriguez D
Istituto di Medicina Legale, Università di Ancona, Policlinico Torrette, Italy.
Forensic Sci Int. 1998 Apr 5;92(2-3):251-8. doi: 10.1016/s0379-0738(98)00021-8.
Blood samples were collected from 100 individuals living in Central Italy and analysed for STR locus D21S11 by capillary electrophoresis on an ABI Prism 310 genetic analyzer. For fragment sizing, PCR amplification products, obtained using a 6-FAM 5'-labeled reverse primer and an unlabeled forward primer, were run with an internal size standard labeled with TAMRA dye and typed using the local reciprocal method. An allele ladder consisting of a mix of sequenced amplified products was also prepared. An Italian population database was established. No deviation from Hardy-Weinberg equilibrium was observed. The result of statistical analysis were highly informative (PD = 0.94; mean exclusion change = 0.66). DNA sequencing was performed on a set of representative alleles by Taq cycle sequencing using dye terminator labeling chemistry. A new structural variant was found.
从居住在意大利中部的100个人身上采集血样，并在ABI Prism 310基因分析仪上通过毛细管电泳对STR基因座D21S11进行分析。对于片段大小测定，使用6-FAM 5'-标记的反向引物和未标记的正向引物获得的PCR扩增产物，与用TAMRA染料标记的内部大小标准一起运行，并使用本地倒数法进行分型。还制备了由测序扩增产物混合物组成的等位基因阶梯。建立了一个意大利人群数据库。未观察到偏离哈迪-温伯格平衡的情况。统计分析结果具有很高的信息量（PD = 0.94；平均排除变化 = 0.66）。通过使用染料终止剂标记化学的Taq循环测序对一组代表性等位基因进行DNA测序。发现了一种新的结构变异体。
