Bell J L, Grassle J P
Institute of Marine and Coastal Sciences, Rutgers State University of New Jersey, New Brunswick 08901-8521, USA.
Mol Mar Biol Biotechnol. 1998 Jun;7(2):127-37.
A molecular DNA probe was developed for identification of larvae of the commercial surfclam Spisula solidissima (family Mactridae), to distinguish early-stage veligers from larvae of other common bivalve species in a study of surfclam settlement and recruitment on the New Jersey continental shelf. For discrimination of S. solidissima from other common bivalve species at the study site (almost all of which belong to different families), an oligonucleotide designed from the nucleotide sequence of the 18S ribosomal RNA gene provides a useful and sensitive family-specific probe and primer. For discrimination between S. solidissima and Mulinia lateralis (both members of Mactridae), the 18S rRNA gene was examined by restriction fragment length polymorphism (RFLP) analysis. A combination of the DNA probe and RFLP analysis provides a positive identification of S. solidissima and M. lateralis larvae in coastal plankton samples.
开发了一种分子DNA探针，用于鉴定商业性冲浪蛤蜊（Spisula solidissima，蛤蜊科）的幼虫，以便在新泽西州大陆架冲浪蛤蜊定居和补充研究中，将早期面盘幼虫与其他常见双壳类物种的幼虫区分开来。为了在研究地点将坚固拟浪蛤与其他常见双壳类物种（几乎所有这些物种都属于不同科）区分开来，根据18S核糖体RNA基因的核苷酸序列设计的寡核苷酸提供了一种有用且灵敏的科特异性探针和引物。为了区分坚固拟浪蛤和侧多齿蛤（均为蛤蜊科成员），通过限制性片段长度多态性（RFLP）分析检测了18S rRNA基因。DNA探针和RFLP分析相结合，可在沿海浮游生物样本中对坚固拟浪蛤和侧多齿蛤幼虫进行阳性鉴定。
