Meulewaeter F, Danthinne X, Van Montagu M, Cornelissen M
Plant Genetic Systems, N.V., Gent, Belgium.
Plant J. 1998 Apr;14(2):169-76. doi: 10.1046/j.1365-313x.1998.00104.x.
The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a 5' cap and a 3' poly(A) tail. The STNV trailer contains an autonomous translational enhancer domain (TED) that promotes translation in vitro by more than one order of magnitude when combined with the 5'-terminal 173 nt of STNV RNA. We now show that the responsible sequence within the 5' region maps to the first 38 nt of the STNV RNA. Mutational analysis indicated that the primary sequence of the STNV 5' 38 nt and TED is important for translation stimulation in vitro, but did not reveal a role for the complementarity between the two. Translation of chimeric STNV-cat RNAs in tobacco protoplasts showed that TED promotes translation in vivo of RNAs lacking a cap and/or a poly(A) tail. Similar to in vitro, TED-dependent translation in tobacco was stimulated further by the STNV 5' 38 nt.
卫星烟草坏死病毒（STNV）的RNA是一种单顺反子信使RNA，既没有5'帽结构也没有3' poly（A）尾。STNV的非编码区包含一个自主翻译增强子结构域（TED），当与STNV RNA的5'末端173个核苷酸结合时，它能在体外将翻译效率提高一个数量级以上。我们现在表明，5'区域内的负责序列定位于STNV RNA的前38个核苷酸。突变分析表明，STNV 5' 38个核苷酸和TED的一级序列对于体外翻译刺激很重要，但未揭示两者之间互补性的作用。烟草原生质体中嵌合STNV-猫RNA的翻译表明，TED能促进缺乏帽结构和/或poly（A）尾的RNA在体内的翻译。与体外情况类似，烟草中依赖TED的翻译在STNV 5' 38个核苷酸的作用下进一步受到刺激。
