卫星烟草坏死病毒翻译增强子结构域的自主功能特征
Features of the autonomous function of the translational enhancer domain of satellite tobacco necrosis virus.
作者信息
Meulewaeter F, Van Montagu M, Cornelissen M
机构信息
Plant Genetic Systems N.V., Universiteit Gent, Belgium.
出版信息
RNA. 1998 Nov;4(11):1347-56. doi: 10.1017/s135583829898092x.
Abstract
The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a cap and a poly(A) tail. Translation of STNV RNA in vitro is promoted by a 120-nt translational enhancer domain (TED) in the 3'-untranslated region. TED also stimulates translation of heterologous mRNAs. In this study, we show that TED stimulates translation of a cat mRNA by increasing translation efficiency to the level of capped mRNA. This stimulatory activity is not impaired by translation through TED. TED stimulates translation efficiency from different positions within the mRNA, varying from the 5' end to 940 nt downstream of the coding region. Duplication of TED has an additive effect on translation stimulation only when located at both ends of the mRNA. On dicistronic RNAs, TED stimulates translation of both cistrons to the same extent. These data suggest that TED acts primarily by recruiting the translational machinery to the RNA.
摘要
卫星烟草坏死病毒（STNV）的RNA是一种单顺反子信使RNA，既没有帽子结构也没有聚腺苷酸尾巴。3'-非翻译区中一个120个核苷酸的翻译增强结构域（TED）可促进STNV RNA在体外的翻译。TED还能刺激异源mRNA的翻译。在本研究中，我们表明TED通过将翻译效率提高到带帽mRNA的水平来刺激猫mRNA的翻译。这种刺激活性不会因通过TED进行翻译而受损。TED可从mRNA内不同位置刺激翻译效率，范围从5'端到编码区下游940个核苷酸处。只有当TED位于mRNA两端时，其重复对翻译刺激才有累加效应。在双顺反子RNA上，TED对两个顺反子的翻译刺激程度相同。这些数据表明，TED主要通过将翻译机制招募到RNA上来发挥作用。
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本文引用的文献
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