Ramchandani S, Bigey P, Szyf M
Department of Pharmacology and Therapeutics, McGill University, Montreal, PQ, Canada.
Biol Chem. 1998 Apr-May;379(4-5):535-40. doi: 10.1515/bchm.1998.379.4-5.535.
We determined the genomic structure of the gene encoding human DNA methyltransferase (DNA MTase). Six overlapping human genomic DNA clones which include all of the known cDNA sequence were isolated. Analysis of these clones demonstrates that the human DNA MTase gene consists of at least 40 exons and 39 introns spanning a distance of 60 kilobases. Elucidation of the chromosomal organization of the human DNA MTase gene provides the template for future structure-function analysis of the properties of mammalian DNA MTase.
我们确定了编码人类DNA甲基转移酶（DNA MTase）的基因的基因组结构。分离出了六个重叠的人类基因组DNA克隆，它们包含了所有已知的cDNA序列。对这些克隆的分析表明，人类DNA MTase基因由至少40个外显子和39个内含子组成，跨越60千碱基的距离。对人类DNA MTase基因染色体组织的阐明为未来对哺乳动物DNA MTase特性进行结构-功能分析提供了模板。
