Vásquez C, Saavedra C, González E
Departamento de Ciencias Biológicas, Universidad de Talca, Chile.
Gene. 1991 Jun 15;102(1):83-5. doi: 10.1016/0378-1119(91)90543-k.
A standard DNA modification methyltransferase (MTase) selection protocol was followed to clone the BstVI restriction and modification system from Bacillus stearothermophilus in Escherichia coli. Both genes were contained in a 4.4-kb EcoRI fragment from B. stearothermophilus V chromosomal DNA. The heterologous expression of these genes did not depend on their orientation in the vector, suggesting that the genes are expressed in E. coli under the control of promoters located on the cloned fragment. Subcloning experiments demonstrated that the bstVIR gene was expressed in the absence of its cognate MTase.
按照标准的DNA修饰甲基转移酶（MTase）选择方案，从嗜热脂肪芽孢杆菌中克隆BstVI限制与修饰系统至大肠杆菌中。这两个基因包含在来自嗜热脂肪芽孢杆菌V染色体DNA的一个4.4 kb的EcoRI片段中。这些基因的异源表达不依赖于它们在载体中的方向，这表明这些基因在位于克隆片段上的启动子控制下在大肠杆菌中表达。亚克隆实验表明bstVIR基因在没有其同源MTase的情况下也能表达。
