从大鼠肝脏线粒体中同时分离出依赖脱氧核糖核酸的核糖核酸聚合酶和聚腺苷酸聚合酶。
Contemporaneous isolation of deoxyribonucleic acid-dependent ribonucleic acid polymerase and poly(A) polymerase from rat liver mitochondria.
作者信息
Gallerani R, Istituto di Ch, Saccone C
出版信息
Biochem J. 1976 Aug 1;157(2):295-300. doi: 10.1042/bj1570295.
Abstract
- Poly(A) polymerase and DNA-dependent RNA polymerase from rat liver mitochondria can be completely separated by using two different chromatographic procedures. 2. Poly(A) polymerase can only incorporate ATP into acid-insoluble material and strongly depends on the addition of an endogenous factor (probably containing a mixture of oligoribonucleotides), but it is not stimulated by DNA. 3. RNA polymerase is fully DNA-dependent and rifampicin-sensitive, but was not stimulated by the endogenous factor mentioned above. 4. The chromatographic behaviour of the two enzymes, together with the properties described, suggest that they represent two different protein molecules.
摘要
- 通过两种不同的色谱方法可以将大鼠肝线粒体中的聚腺苷酸聚合酶和依赖DNA的RNA聚合酶完全分离。2. 聚腺苷酸聚合酶只能将ATP掺入酸不溶性物质中，并且强烈依赖于内源性因子（可能含有寡核糖核苷酸混合物）的添加，但不受DNA刺激。3. RNA聚合酶完全依赖DNA且对利福平敏感，但不受上述内源性因子刺激。4. 这两种酶的色谱行为以及所描述的特性表明它们代表两种不同的蛋白质分子。
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