Paulin F E, Chappell S A, Willis A E
Department of Biochemistry, University of Leicester, University Road, Leicester LE1 7RH, UK.
Nucleic Acids Res. 1998 Jul 1;26(13):3097-103. doi: 10.1093/nar/26.13.3097.
A 340 nucleotide section of the c- myc 5' untranslated region (UTR) contains an internal ribosome entry segment. We have described previously a mutation in this region of RNA in cell lines derived from patients with multiple myeloma (MM) which exhibit increased expression of c- myc protein by an aberrant translational mechanism. In this study we show by electrophoretic mobility shift assays (EMSA), north-western blotting and UV cross-linking that radiolabelled c- myc 5' UTR RNA transcripts which harbour the mutation cause enhanced binding of cellular proteins. In addition, we also demonstrate that an MM derived cell line possesses an altered repertoire of RNA binding proteins. Our data suggest that the deregulated expression of c -myc in MM could result both from the effect of the mutation and the additional proteins which are present in these cell types.
c-myc 5'非翻译区（UTR）的一段340个核苷酸的区域包含一个内部核糖体进入片段。我们之前已经描述过，在源自多发性骨髓瘤（MM）患者的细胞系中，该RNA区域存在一种突变，这些细胞系通过异常翻译机制表现出c-myc蛋白表达增加。在本研究中，我们通过电泳迁移率变动分析（EMSA）、西北印迹法和紫外线交联法表明，携带该突变的放射性标记的c-myc 5' UTR RNA转录本会导致细胞蛋白结合增强。此外，我们还证明，一个源自MM的细胞系拥有改变的RNA结合蛋白库。我们的数据表明，MM中c-myc的表达失调可能是由突变的影响以及这些细胞类型中存在的其他蛋白质共同导致的。
