蝗虫微孢子虫中的U2和U6小核仁RNA基因：功能性剪接体的证据
U2 and U6 snRNA genes in the microsporidian Nosema locustae: evidence for a functional spliceosome.
作者信息
Fast N M, Roger A J, Richardson C A, Doolittle W F
机构信息
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada.
出版信息
Nucleic Acids Res. 1998 Jul 1;26(13):3202-7. doi: 10.1093/nar/26.13.3202.
The removal of introns from pre-messenger RNA is mediated by the spliceosome, a large complex composed of many proteins and five small nuclear RNAs (snRNAs). Of the snRNAs, the U6 and U2 snRNAs are the most conserved in sequence, as they interact extensively with each other and also with the intron, in several base pairings that are necessary for splicing. We have isolated and sequenced the genes encoding both U6 and U2 snRNAs from the intracellularly parasitic microsporidian Nosema locustae . Both genes are expressed. Both RNAs can be folded into secondary structures typical of other known U6 and U2 snRNAs. In addition, the N.locustae U6 and U2 snRNAs have the potential to base pair in the functional intermolecular interactions that have been characterized by extensive analyses in yeast and mammalian systems. These results indicate that the N.locustae U6 and U2 snRNAs may be functional components of an active spliceosome, even though introns have not yet been found in microsporidian genes.
前体信使RNA中内含子的去除是由剪接体介导的，剪接体是一种由许多蛋白质和五种小核RNA（snRNA）组成的大型复合体。在这些snRNA中，U6和U2 snRNA在序列上最为保守，因为它们彼此之间以及与内含子广泛相互作用，形成了剪接所需的几种碱基对。我们从细胞内寄生的微孢子虫蝗虫微孢子虫中分离并测序了编码U6和U2 snRNA的基因。这两个基因均表达。两种RNA都可以折叠成其他已知U6和U2 snRNA典型的二级结构。此外，蝗虫微孢子虫U6和U2 snRNA有可能在酵母和哺乳动物系统中经过广泛分析所确定的功能性分子间相互作用中形成碱基对。这些结果表明，蝗虫微孢子虫U6和U2 snRNA可能是活性剪接体的功能成分，尽管在微孢子虫基因中尚未发现内含子。
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