胰岛素和糖皮质激素对体外培养的大鼠脂肪组织中磷酸烯醇式丙酮酸羧激酶（GTP）合成与降解的影响。
The effect of insulin and glucocorticoids on the synthesis and degradation of phosphoenolpyruvate carboxykinase (GTP) in rat adipose tissue cultured in vitro.
作者信息
Meyuhas O, Reshef L, Ballard F J, Hanson R W
出版信息
Biochem J. 1976 Jul 15;158(1):9-16. doi: 10.1042/bj1580009.
Abstract
- Epididymal adipose tissue from the rat was maintained in culture for periods of up to 96h. 2. After an initial decrease in protein synthesis during the first 24h of culture, the adipose tissue recovered its capacity to synthesize and accumulate proteins of a relatively large size. 3. The activity of phosphoenolpyruvate carboxykinase decreased in a parallel manner, but increased again after 24h of incubation of the tissue in culture, to a value twice that noted in the tissue in vivo. This increase in enzyme activity was due to an increase in its rate of synthesis. 4. Both insulin and dexamethasone (9alpha-fluoro-16alpha-methyl-11beta,17,-21-trihydroxypregna-1,4-diene-3,20-dione) inhibited phosphoenolpyruvate carboxykinase synthesis, but dexamethasone also decreased total protein synthesis. 5. The half-life of phosphoenolpyruvate carboxykinase in adipose tissue cultured in vitro was 5--7h and was not altered by insulin or dexamethasone. 6. It is concluded that both insulin and glucocroticoids lower the activity of phosphoenolpyruvate carboxykinase in rat adipose tissue by decreasing its rate of synthesis.
摘要
- 将大鼠附睾脂肪组织培养长达96小时。2. 在培养的最初24小时内蛋白质合成出现初步下降后，脂肪组织恢复了合成和积累相对大分子蛋白质的能力。3. 磷酸烯醇丙酮酸羧激酶的活性以平行方式下降，但在组织培养孵育24小时后再次升高，达到体内组织中所记录值的两倍。这种酶活性的增加是由于其合成速率的提高。4. 胰岛素和地塞米松（9α-氟-16α-甲基-11β,17,21-三羟基孕甾-1,4-二烯-3,20-二酮）均抑制磷酸烯醇丙酮酸羧激酶的合成，但地塞米松也降低总蛋白质合成。5. 体外培养的脂肪组织中磷酸烯醇丙酮酸羧激酶的半衰期为5 - 7小时，且不受胰岛素或地塞米松的影响。6. 得出的结论是，胰岛素和糖皮质激素均通过降低磷酸烯醇丙酮酸羧激酶的合成速率来降低大鼠脂肪组织中该酶的活性。
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