肝脏细胞质中新形成的游离小核糖体亚基与循环利用的游离小核糖体亚基之间的差异。
Differences between newly formed and recycled free small ribosome subunits in liver cytoplasm.
作者信息
Hinton R H, Mullock B M
出版信息
Biochem J. 1976 Jul 15;158(1):97-103. doi: 10.1042/bj1580097.
Abstract
Heterogeneity among the free small ribosomal subunits of rat liver was studied. Newly made free small subunits differ from the bulk of the free subunits in the following ways. (1) The newly made free small subunits sediment slightly faster than most free small subunits and form a band at slightly lower densities. (2) Mature free small subunits, prepared under conditions which minimize the amount of loosely bound protein, readily dimerize in solutions of low ionic strength. Little dimerization is found with newly formed subunits. This permits the separation of fractions enriched in newly formed subunits. (3) The fraction enriched in newly formed subunits contains a distinctive additional protein as compared the most of the free small subunits.
摘要
对大鼠肝脏游离小核糖体亚基的异质性进行了研究。新合成的游离小亚基与大部分游离亚基在以下方面存在差异。（1）新合成的游离小亚基沉降速度比大多数游离小亚基略快，且在密度略低的位置形成一条带。（2）在使松散结合蛋白量最小化的条件下制备的成熟游离小亚基，在低离子强度溶液中很容易二聚化。新形成的亚基很少发生二聚化。这使得富含新形成亚基的组分得以分离。（3）与大多数游离小亚基相比，富含新形成亚基的组分含有一种独特的额外蛋白质。
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