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Biochem J. 1976 Jul 15;158(1):97-103. doi: 10.1042/bj1580097.
2
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Mol Biochem Parasitol. 1984 Jul;12(3):249-60. doi: 10.1016/0166-6851(84)90082-3.
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[Various characteristics of the protein composition of ribosomes separated from granular membranes of the endoplasmic reticulum of rat liver].[从大鼠肝脏内质网颗粒膜分离出的核糖体蛋白质组成的各种特征]
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引用本文的文献

1
Heterogeneity of the translational machinery: Variations on a common theme.翻译机制的异质性：同一主题的变体
Biochimie. 2015 Jul;114:39-47. doi: 10.1016/j.biochi.2014.12.011. Epub 2014 Dec 24.

本文引用的文献

1
Buoyant densities of cytoplasmic ribonucleoprotein particles of mammalian cells: distinctive character of ribosome subunits and the rapidly labeled components.哺乳动物细胞胞质核糖核蛋白颗粒的浮力密度：核糖体亚基和快速标记成分的独特特征。
J Mol Biol. 1966 Apr;16(2):255-68. doi: 10.1016/s0022-2836(66)80171-7.
2
Characterization of rapidly labeled 40-S ribonucleoprotein particles in rat liver cytoplasm.大鼠肝细胞质中快速标记的40-S核糖核蛋白颗粒的特性分析。
Biochim Biophys Acta. 1971 Apr 29;238(1):139-49. doi: 10.1016/0005-2787(71)90017-7.
3
The isolation of polysomes from rat liver. Contamination by membrane proteins with high affinity to ribosomes.从大鼠肝脏中分离多核糖体。受与核糖体具有高亲和力的膜蛋白污染。
Biochim Biophys Acta. 1971 Apr 8;232(4):705-16.
4
Subribosomal particles separated from liver and hepatoma homogenates with a zonal rotor.用区带转头从肝脏和肝癌匀浆中分离出的亚核糖体颗粒。
Eur J Biochem. 1971 Feb;18(4):485-95. doi: 10.1111/j.1432-1033.1971.tb01268.x.
5
Rapidly labeled, polydisperse RNA in rat-liver cytoplasm: evidence that it is contained in ribonucleoprotein particles of heterogeneous size.大鼠肝脏细胞质中快速标记的多分散RNA：证据表明其存在于大小各异的核糖核蛋白颗粒中。
Biochim Biophys Acta. 1970 Feb 18;199(2):405-20. doi: 10.1016/0005-2787(70)90083-3.
6
[Preparation of rat liver polyribosomes in large quantities].[大鼠肝脏多核糖体的大量制备]
Biokhimiia. 1969 Jul-Aug;34(4):839-48.
7
The role of ribosomal subunits in mammalian cells.核糖体亚基在哺乳动物细胞中的作用。
Cold Spring Harb Symp Quant Biol. 1969;34:555-65. doi: 10.1101/sqb.1969.034.01.063.
8
Enumeration of rabbit reticulocyte ribosomal proteins.兔网织红细胞核糖体蛋白的计数
J Mol Biol. 1971 Dec 14;62(2):403-5. doi: 10.1016/0022-2836(71)90435-9.
9
Two separable cytoplasmic pools of native ribosomal subunits in chick embryo tissue culture cells.鸡胚组织培养细胞中天然核糖体亚基的两个可分离的细胞质池。
Biochim Biophys Acta. 1972 May 10;269(2):265-75. doi: 10.1016/0005-2787(72)90436-4.
10
Molecular weight distribution of proteins in rabbit reticulocyte ribosomal subunits.兔网织红细胞核糖体亚基中蛋白质的分子量分布
J Mol Biol. 1971 Oct 14;61(1):143-56. doi: 10.1016/0022-2836(71)90212-9.

肝脏细胞质中新形成的游离小核糖体亚基与循环利用的游离小核糖体亚基之间的差异。

Differences between newly formed and recycled free small ribosome subunits in liver cytoplasm.

作者信息

Hinton R H, Mullock B M

出版信息

Biochem J. 1976 Jul 15;158(1):97-103. doi: 10.1042/bj1580097.

DOI:10.1042/bj1580097
PMID:962894
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163941/
Abstract

Heterogeneity among the free small ribosomal subunits of rat liver was studied. Newly made free small subunits differ from the bulk of the free subunits in the following ways. (1) The newly made free small subunits sediment slightly faster than most free small subunits and form a band at slightly lower densities. (2) Mature free small subunits, prepared under conditions which minimize the amount of loosely bound protein, readily dimerize in solutions of low ionic strength. Little dimerization is found with newly formed subunits. This permits the separation of fractions enriched in newly formed subunits. (3) The fraction enriched in newly formed subunits contains a distinctive additional protein as compared the most of the free small subunits.

摘要

对大鼠肝脏游离小核糖体亚基的异质性进行了研究。新合成的游离小亚基与大部分游离亚基在以下方面存在差异。（1）新合成的游离小亚基沉降速度比大多数游离小亚基略快，且在密度略低的位置形成一条带。（2）在使松散结合蛋白量最小化的条件下制备的成熟游离小亚基，在低离子强度溶液中很容易二聚化。新形成的亚基很少发生二聚化。这使得富含新形成亚基的组分得以分离。（3）与大多数游离小亚基相比，富含新形成亚基的组分含有一种独特的额外蛋白质。