通过扩增产物长度多态性（APLP）分析对乙醇脱氢酶2（ADH2）和乙醛脱氢酶2（ALDH2）基因座进行同步基因分型。
Simultaneous genotyping of alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) loci by amplified product length polymorphism (APLP) analysis.
作者信息
Aoshima T, Umetsu K, Yuasa I, Watanabe G, Suzuki T
机构信息
Department of Forensic Medicine, Yamagata University School of Medicine, Japan.
出版信息
Electrophoresis. 1998 May;19(5):659-60. doi: 10.1002/elps.1150190508.
Genotyping of the alcohol dehydrogenase 2 (ADH2) and aldehyde dehydrogenase 2 (ALDH2) loci is important in alcohol studies. We describe a method for simultaneous genotyping of ADH2 and ALDH2 based on amplified product length polymorphism (APLP) analysis. Two polymerase chain reaction (PCR) fragments for ADH2 (57 bp, 53 bp) and two for ALDH2 (78 bp, 73 bp) are simultaneously amplified. Nine banding patterns reflecting the genotypes of the ADH2 and ALDH2 loci are clearly and unambiguously distinguished. The APLP method seems to be particularly suited for large-scale population studies of ADH2 and ALDH2 loci because it is simple and rapid.
酒精脱氢酶2（ADH2）和乙醛脱氢酶2（ALDH2）基因座的基因分型在酒精研究中很重要。我们描述了一种基于扩增产物长度多态性（APLP）分析同时对ADH2和ALDH2进行基因分型的方法。用于ADH2的两个聚合酶链反应（PCR）片段（57 bp，53 bp）和用于ALDH2的两个片段（78 bp，73 bp）同时被扩增。清晰且明确地区分出反映ADH2和ALDH2基因座基因型的九种条带模式。APLP方法似乎特别适用于对ADH2和ALDH2基因座进行大规模人群研究，因为它简单快速。
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