Rajyaguru J M, Torres D S, Abel E, Richardson M C, Muszynski M J
Department of Pediatric Infectious Diseases Research, Nemours Childrens Clinic-Orlando, FL 32806, USA.
J Antimicrob Chemother. 1998 May;41(5):557-61. doi: 10.1093/jac/41.5.557.
Aminoglycosides disrupt the outer membrane of Pseudomonas aeruginosa to facilitate access to their intracellular target. High-resolution X-ray micrography of live specimens is a relatively new technique. We used laser (nanosecond) plasma to image live cells of P. aeruginosa ATCC 27853. After exposure to 25 mg/L gentamicin for 15 min. we observed perturbation of the cell surface, membrane blebbings (370 nm and 273 nm diameter) away from the cell, formation of distinct channels (241 nm long) resulting from indentation and induction of cell elongation from 3-3.6 microm (control) to 4.6-5.26 microm (gentamicin-treated cells). These data illustrate the potential of high-resolution X-ray micrography for studying effects of drugs on live microbiological specimens.
氨基糖苷类药物破坏铜绿假单胞菌的外膜，以便接近其细胞内靶点。对活标本进行高分辨率X射线显微照相是一项相对较新的技术。我们使用激光（纳秒）等离子体对铜绿假单胞菌ATCC 27853的活细胞进行成像。在暴露于25mg/L庆大霉素15分钟后，我们观察到细胞表面受到扰动，细胞膜从细胞表面鼓起（直径分别为370nm和273nm），由于压痕形成了明显的通道（长241nm），并且细胞从3 - 3.6微米（对照）伸长至4.6 - 5.26微米（庆大霉素处理的细胞）。这些数据说明了高分辨率X射线显微照相在研究药物对活微生物标本影响方面的潜力。
