Luhr O R, Frostell C G, Heywood R, Riley S, Lönnqvist P
Division of Anaesthesia and Intensive care, Karolinska Institute at Danderyd Hospital, Stockholm, Sweden.
Mutat Res. 1998 May 11;414(1-3):107-15. doi: 10.1016/s1383-5718(98)00044-8.
inhalation of nitric oxide (INO) leads to vasodilation of pulmonary vasculature in ventilated regions of the lung. The clinical use of INO, although not formally approved as a drug, is widespread. NO may rapidly form nitrogen dioxide (NO2) in an oxygen containing gas mixture. NO2 has been shown to induce chromosome aberrations and mutations in both animal and bacterial test systems. We investigated whether a 2-h exposure to NO would increase frequencies of cells with chromosome aberrations in peripheral blood lymphocytes of human volunteers.
10 volunteers were exposed to inhaled NO 40 parts per million (ppm) for 2 h. Pre- and post-exposure blood samples were analysed.
no statistically significant differences (p</=0.05) in chromosome aberrations were observed between pre- and post-exposure samples.
no detectable increase of chromosome aberrations in human peripheral blood lymphocytes after 2 h of NO-inhalation 40 ppm was found.
吸入一氧化氮（INO）可导致肺通气区域的肺血管舒张。尽管INO未被正式批准为药物，但其临床应用却很广泛。在含氧气的气体混合物中，NO可能会迅速形成二氧化氮（NO2）。在动物和细菌测试系统中，NO2已被证明可诱导染色体畸变和突变。我们调查了人类志愿者外周血淋巴细胞经2小时NO暴露后，具有染色体畸变的细胞频率是否会增加。
10名志愿者吸入百万分之40（ppm）的NO，持续2小时。对暴露前后的血样进行分析。
暴露前后样本之间未观察到染色体畸变有统计学显著差异（p≤0.05）。
发现吸入40 ppm NO 2小时后，人类外周血淋巴细胞中染色体畸变未出现可检测到的增加。
