根癌农杆菌介导的玉米（Zea mays L.）高效转化

High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens.

作者信息

Ishida Y, Saito H, Ohta S, Hiei Y, Komari T, Kumashiro T

机构信息

Plant Breeding and Genetics Research Laboratory, Japan Tobacco Inc., Shizuoka, Japan.

出版信息

Nat Biotechnol. 1996 Jun;14(6):745-50. doi: 10.1038/nbt0696-745.

DOI:10.1038/nbt0696-745

PMID:9630983

Abstract

Transformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried "super-binary" vectors. Frequencies of transformation (independent transgenic plants/embryos) were between 5% and 30%. Almost all transformants were normal in morphology, and more than 70% were fertile. Stable integration, expression, and inheritance of the transgenes were confirmed by molecular and genetic analysis. Between one and three copies of the transgenes were integrated with little rearrangement, and the boundaries of T-DNA were similar to those in transgenic dicotyledons and rice. F1 hybrids between A188 and five other inbreds were transformed at low frequencies.

摘要

将玉米自交系A188的未成熟胚与携带“超级双元”载体的根癌农杆菌共培养，高效产生了转化体。转化频率（独立转基因植株/胚）在5%至30%之间。几乎所有转化体形态正常，70%以上可育。通过分子和遗传分析证实了转基因的稳定整合、表达和遗传。转基因以很少的重排整合了一至三个拷贝，T-DNA的边界与转基因双子叶植物和水稻中的相似。A188与其他五个自交系之间的F1杂种转化频率较低。