Jansen S, Kriegesmann B, Brenig B
Tierarztliches Institut, Universitat Gottingen Abteilung Molekularbiologie, Germany.
Biotechniques. 1998 Jun;24(6):998-1001. doi: 10.2144/98246bc02.
Here, we present a Microsoft Windows application that has been written to emulate a microplate reader. After scanning a standard 96-well microplate, color gradations in the slots can be analyzed quantitatively and be used for the estimation of enzyme kinetics, binding assays or concentration determination. In a test, series accuracy of the program was determined. We show that the returned optical densities correlated highly with the true concentrations (r > 0.97). Thus, the software is useful for a wide number of applications. Further, the program is easy to handle and is available free of charge.
在此，我们展示了一个为模拟酶标仪而编写的Microsoft Windows应用程序。扫描标准的96孔微孔板后，孔槽中的颜色渐变可进行定量分析，并用于酶动力学、结合分析或浓度测定的评估。在一次测试中，确定了该程序的系列准确性。我们发现返回的光密度与真实浓度高度相关（r > 0.97）。因此，该软件可用于多种应用。此外，该程序易于操作且免费提供。
