Brooks S P
Nutrition Research Division, Health Canada 3W Banting Research Centre, Ottawa, Ontario.
Biotechniques. 1994 Dec;17(6):1154-61.
A computer program (microplate analysis [MPA] program) is described that allows manipulation of kinetic data obtained from a microplate reader. It is particularly useful for obtaining enzyme velocities from microplate data. Data can be displayed either as an 8 x 12 grid which shows all microplate wells at low resolution or as single enzyme progress curves which show the reaction time course in greater detail and allow data manipulation (single curve mode). In the single curve mode, the X-axis of the progress curves can be displayed either as discrete time points numbered from the first to the last or as real time in seconds. The program can calculate the maximum, minimum or initial reaction rate by either linear or robust regression. In the single curve mode, the user can control the range of data points included in the regression analysis and the number of data points over which the rate is averaged. In this way, the maximum (or minimum) rate within a specific range of data can be determined. The major advantage of the MPA program is that the range settings are specific to each well. This gives the user complete control over the presentation and calculation of the kinetic data. The MPA program requires an AS-CII file containing data blocks of 96 sequential absorbance readings for each time point and the time interval between successive absorbance readings.
本文描述了一种计算机程序（微孔板分析[MPA]程序），该程序可用于处理从微孔板读数器获得的动力学数据。它对于从微孔板数据中获取酶活性速度特别有用。数据可以以8×12网格的形式显示，该网格以低分辨率显示所有微孔板孔，也可以以单个酶反应进程曲线的形式显示，该曲线更详细地显示反应时间进程并允许进行数据处理（单曲线模式）。在单曲线模式下，反应进程曲线的X轴可以显示为从第一个到最后一个编号的离散时间点，也可以显示为以秒为单位的实际时间。该程序可以通过线性回归或稳健回归计算最大、最小或初始反应速率。在单曲线模式下，用户可以控制回归分析中包含的数据点范围以及用于平均速率的数据点数量。通过这种方式，可以确定特定数据范围内的最大（或最小）速率。MPA程序的主要优点是范围设置特定于每个孔。这使用户能够完全控制动力学数据的呈现和计算。MPA程序需要一个ASCII文件，该文件包含每个时间点96个连续吸光度读数的数据块以及连续吸光度读数之间的时间间隔。
