Cha C I, Kim J M, Shin D H, Kim Y S, Kim J, Gurney M E, Lee K W
Department of Anatomy, College of Medicine, Seoul National University.
Neuroreport. 1998 May 11;9(7):1503-6. doi: 10.1097/00001756-199805110-00047.
The distribution of the neuronal isoform of nitric oxide synthase (nNOS) in the spinal cord of transgenic mice expressing a mutated human copper/zinc superoxide dismutase gene was enhanced when investigated by immunocytochemistry. Immunocytochemistry showed intensely stained NOS-immunoreactive (IR) glial cells with the appearance of astrocytes in the spinal cord and brain stem of transgenic mice, but none were observed at these sites in control mice. Using antisera directed against GFAP, the specific marker for astrocyte, the glial cells were confirmed by immunocytochemistry to be astrocytes. This immunocytochemical evidence suggests that nitric oxide may mediate glutamate neurotoxicity, and this study provides the first in vivo evidence that nitric oxide may be implicated in the pathologic process of human familial amyotrophic lateral sclerosis.
通过免疫细胞化学研究发现，在表达突变型人铜/锌超氧化物歧化酶基因的转基因小鼠脊髓中，一氧化氮合酶（nNOS）的神经元亚型分布增强。免疫细胞化学显示，转基因小鼠脊髓和脑干中出现了强烈染色的一氧化氮合酶免疫反应性（IR）神经胶质细胞，其形态类似星形胶质细胞，而在对照小鼠的这些部位未观察到此类细胞。使用针对星形胶质细胞特异性标志物胶质纤维酸性蛋白（GFAP）的抗血清，通过免疫细胞化学证实这些神经胶质细胞为星形胶质细胞。这一免疫细胞化学证据表明一氧化氮可能介导谷氨酸神经毒性，并且该研究首次提供了体内证据，证明一氧化氮可能参与人类家族性肌萎缩侧索硬化症的病理过程。
