Sedzik J, Kotake Y, Uyemura K
Department of Physiology, Keio University School of Medicine, Tokyo, Japan.
Neuroreport. 1998 May 11;9(7):1595-600. doi: 10.1097/00001756-199805110-00062.
PASII/PMP22 protein (mol. wt 22 kDa, pI 8.8) is an abundant and extremely hydrophobic glycoprotein of PNS myelin which is solubilized effectively by SDS. In humans, this protein is involved in hereditary neuropathies. Here we describe a simple method for purification of PASII/PMP22, suitable for crystallization trials. We usually obtained 10-20 mg PASII/PMP22 from 10 g bovine spinal roots. It is notable, that the original protocol was designated for purification of P0 myelin glycoprotein, and purification of PASII/PMP22 is a bonus. Extensive crystallization trials are in progress.
PASII/PMP22蛋白（分子量22 kDa，等电点8.8）是周围神经系统髓磷脂中一种丰富且极具疏水性的糖蛋白，可被十二烷基硫酸钠有效溶解。在人类中，这种蛋白与遗传性神经病变有关。在此，我们描述了一种纯化PASII/PMP22的简单方法，适用于结晶试验。我们通常从10克牛脊髓神经根中获得10 - 20毫克PASII/PMP22。值得注意的是，最初的方案是用于纯化P0髓磷脂糖蛋白的，而PASII/PMP22的纯化算是意外收获。大量的结晶试验正在进行中。
