Ridpath J F, Bolin S R
Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA 50010, USA.
Mol Cell Probes. 1998 Apr;12(2):101-6. doi: 10.1006/mcpr.1998.0158.
There are two genotypes among bovine viral diarrhoea viruses (BVDV), BVDV1 and BVDV2. Within the BVDV1 genotype there are two distinct subgenotypes, BVD1a and BVD1b. Serology and monoclonal antibody binding are used to differentiate BVDV from classical swine fever virus (CSFV) and border disease virus (BDV), the other members of the Pestivirus genus. These techniques are less useful in the differentiation and segregation of viruses within the BVDV species. In this study, differential polymerase chain reaction (PCR) amplification has been evaluated as a tool for segregating BVDV isolates into genotypes and subgenotypes. Polymerase chain reaction primers were selected based on the comparison of 5' untranslated region sequences from CSVF, BDV, BVDV1a, BVDV1b and BVDV2. Differential PCR tests were validated using 345 viruses isolated from cattle and small ruminants that had previously been segregated into genotypes and subgenotypes. There was 100% correlation between segregation by differential PCR and the previous segregation of these viral isolates.
牛病毒性腹泻病毒（BVDV）存在两种基因型，即BVDV1和BVDV2。在BVDV1基因型中，有两种不同的亚基因型，即BVD1a和BVD1b。血清学和单克隆抗体结合用于区分BVDV与瘟病毒属的其他成员，即经典猪瘟病毒（CSFV）和边境病病毒（BDV）。这些技术在区分BVDV物种内的病毒方面作用较小。在本研究中，差异聚合酶链反应（PCR）扩增已被评估为一种将BVDV分离株分为基因型和亚基因型的工具。基于对CSFV、BDV、BVDV1a、BVDV1b和BVDV2的5'非翻译区序列的比较选择聚合酶链反应引物。使用从牛和小反刍动物中分离的345种病毒进行差异PCR测试验证，这些病毒先前已被分为基因型和亚基因型。差异PCR的分类与这些病毒分离株先前的分类之间存在100%的相关性。
