Fliegerová K, Benada O, Flint H J
Institute of Animal Physiology and Genetics, Academy of Sciences of Czech Republic, Prague, Czech Republic.
Lett Appl Microbiol. 1998 Apr;26(4):243-7. doi: 10.1046/j.1472-765x.1998.00299.x.
The plasmid content of six different isolates of Selenomonas ruminantium from the rumen of sheep, cows or goats was examined by electron microscopy. In addition to small plasmids (< 12 kb) studied previously, all six strains contained at least one plasmid larger than 20 kb. Plasmid sizes of 1.4, 2.1, 2.4, 5.0, 6.2, 20.4, 20.8, 22.7, 23.3, 29.3, 30.7, 34.4 and 42.6 kb were estimated from contour length measurements. DNA-DNA hybridization experiments revealed homology among the large plasmids from five strains, while the 20.8 kb plasmid from a sixth isolate showed no apparent relationship with the plasmids of the other strains.
通过电子显微镜检查了从绵羊、奶牛或山羊瘤胃中分离出的六种不同的反刍月形单胞菌菌株的质粒含量。除了先前研究过的小质粒（<12 kb）外，所有六个菌株都至少含有一个大于20 kb的质粒。根据轮廓长度测量估计质粒大小为1.4、2.1、2.4、5.0、6.2、20.4、20.8、22.7、23.3、29.3、30.7、34.4和42.6 kb。DNA-DNA杂交实验揭示了五个菌株的大质粒之间存在同源性，而第六个分离株的20.8 kb质粒与其他菌株的质粒没有明显关系。
