Yin H S, Lee L H
Department of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan, Republic of China.
J Gen Virol. 1998 Jun;79 ( Pt 6):1411-3. doi: 10.1099/0022-1317-79-6-1411.
Cytoplasmic extracts prepared from avian reovirus (ARV) strain S1133-infected chicken embryo fibroblasts were examined for the presence of RNA-binding proteins in order to identify and characterize ARV RNA-binding proteins. Analysis of binding activity to poly(A)-Sepharose indicated that infected cells contained significant amounts of a protein that co-migrated with ARV protein sigmaNS present in total virus-infected cell extracts. Determination of the N-terminal amino acid sequence of several peptide fragments generated by V8 protease digestion of the poly(A)-Sepharose-purified protein confirmed that this viral protein was sigmaNS. Competition assays showed that single-stranded RNA from the unrelated avian pathogen infectious bursal disease virus was able to compete for binding of sigmaNS to poly(A)-Sepharose. These data suggest that ARV sigmaNS binds to single-stranded RNA in a nucleotide sequence non-specific manner and is functionally similar to its counterpart specified by mammalian reovirus.
为了鉴定和表征禽呼肠孤病毒（ARV）的RNA结合蛋白，对从感染ARV S1133株的鸡胚成纤维细胞制备的细胞质提取物进行了RNA结合蛋白检测。对与聚（A）-琼脂糖的结合活性分析表明，感染细胞含有大量与总病毒感染细胞提取物中存在的ARV蛋白sigmaNS共迁移的蛋白质。对聚（A）-琼脂糖纯化蛋白经V8蛋白酶消化产生的几个肽片段的N端氨基酸序列测定证实，这种病毒蛋白是sigmaNS。竞争试验表明，来自无关禽病原体传染性法氏囊病病毒的单链RNA能够竞争sigmaNS与聚（A）-琼脂糖的结合。这些数据表明，ARV sigmaNS以核苷酸序列非特异性方式结合单链RNA，并且在功能上与其哺乳动物呼肠孤病毒对应物相似。
