Claydon M A, Davey S N, Edwards-Jones V, Gordon D B
Department of Biological Sciences, Manchester Metropolitan University, UK.
Nat Biotechnol. 1996 Nov;14(11):1584-6. doi: 10.1038/nbt1196-1584.
Antibiotic-resistant strains of bacteria continue to emerge, increasing the need for their fast and accurate identification. Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), has become a prominent technique in biological mass spectrometry. We report the application of MALDI-TOF-MS for the identification of intact Gram-negative and Gram-positive microorganisms taken directly from culture. Analysis of bacteria from a single colony is possible, allowing the screening of mixed cultures. Sample preparation is simple and the analysis automated, providing spectra within minutes. The spectra obtained allow identification of microorganisms from different genera, different species, and from different strains of the same species. The procedure provides a unique mass spectral fingerprint of the microorganism, produced from desorbed components of the cell wall. Consistent data were obtained from subcultures grown for 3-day and 6-day periods, from the same cultures 1 day later and from fresh subcultures 2 months later.
抗生素耐药性细菌菌株不断出现，这增加了对其进行快速准确鉴定的需求。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）已成为生物质谱中的一项突出技术。我们报告了MALDI-TOF-MS在直接从培养物中获取的完整革兰氏阴性和革兰氏阳性微生物鉴定中的应用。对单个菌落中的细菌进行分析是可行的，从而能够对混合培养物进行筛选。样品制备简单且分析自动化，几分钟内即可提供光谱。所获得的光谱能够鉴定来自不同属、不同种以及同一种不同菌株的微生物。该方法提供了由细胞壁解吸成分产生的微生物独特质谱指纹。从培养3天和6天的传代培养物、1天后的同一培养物以及2个月后的新鲜传代培养物中均获得了一致的数据。
