Bukowska B, Reszka E, Duda W
Department of Biophysics Environmental Polution, University of Lódź, Poland.
Biochem Mol Biol Int. 1998 Jun;45(1):47-59. doi: 10.1080/15216549800202422.
The effect of phenoxyherbicides and their metabolites on the structure of oxy- and deoxyhemoglobin was studied by using different doses and times of incubation of hemoglobin with the herbicide. It was ascertained that among the investigated hemoglobins the most sensitive was carp oxyhemoglobin incubated with 2,4-D (2,4-dichlorophenoxyacetic acid) and the least sensitive was human hemoglobin. Comparing the toxicity of 2,4-D, MCPA (2-methyl-4-chlorophenoxyacetic acid), 2,4-DCP (2,4-dichlorophenol), 2,4-DMP (2,4-dimethylphenol) it was found that the highest decrease occurred in bovine hemoglobin incubated with 2,4-DMP. The phenoxyherbicides caused stabilization of the structure of T-deoxyhemoglobin in vitro, in that they decreased the oxygen affinity with a simultaneous increase in methemoglobin concentration.
通过使用不同剂量的除草剂以及血红蛋白与除草剂不同的孵育时间，研究了苯氧基除草剂及其代谢产物对氧合血红蛋白和脱氧血红蛋白结构的影响。结果确定，在所研究的血红蛋白中，与2,4 -二氯苯氧乙酸（2,4-D）孵育的鲤鱼氧合血红蛋白最为敏感，而人血红蛋白最不敏感。比较2,4-D、2-甲基-4-氯苯氧乙酸（MCPA）、2,4-二氯苯酚（2,4-DCP）和2,4-二甲基苯酚（2,4-DMP）的毒性发现，与2,4-DMP孵育的牛血红蛋白下降幅度最大。苯氧基除草剂在体外会导致T型脱氧血红蛋白结构的稳定，因为它们降低了氧亲和力，同时使高铁血红蛋白浓度增加。
