Vibert J F, Bertrand F, Denavit-Saubié M, Hugelin A
Brain Res. 1976 Sep 17;114(2):211-25. doi: 10.1016/0006-8993(76)90667-3.
Respiration related units (RRU) were recorded during a stratigraphic exploration of medulla and pons from the cervical junction to the caudal part of the pneumotaxic system in the semi-chronic locally anesthetized 'isolated respiratory centre' of the cat. Metal 'low impedance, capacitance compensated' microelectrodes recorded multi-unit signals from which unitary discharges were discriminated and processed by computer; it is suggested that using these techniques, the sample was a good representation of the total unit population. The phase relation to phrenic discharge was determined on cycle triggered time histograms. Of 23,000 units, 28% had a definite respiratory modulation. Examined individually, each RRU showed a stable discharge pattern corresponding to one of various respiratory types, the majority of which have been described previously. Both temporal and spatial distributions of RRU discharges were analyzed. Temporal distribution of peak firing frequencies (PFF) of 5,000 RRU sampled anatomically at random showed two main populations whose modes were observed during inspiration (I) or expiration (E). Troughs were observed in the histogram at the transition from I to E and E to I, thus indicating low probability for finding phase spanning RRU in the medulla and pons up to the pneumotaxic level. These statistical results turned out to be identical to those obtained with an a priori classification method comparable to that used in most of the previous works. In addition, the PFF distribution suggested that the E population could be further divided into 3 sub-populations whose modes fall in early, mid, and late expiration respectively. Comparison of RRU temporal distribution in two regions, one rostral, another caudal to a frontal Horsley-Clarke plane situated 3 mm in front of the obex, showed that, in the caudal region, 70% of the RRU were I units, while, in the rostral medulla and pons, equal proportions of I and E neurons were found. Temporal distribution of RRU peak frequencies was studied separately in anatomical structures where the probability of finding RRU was high. No clear correspondence between RRU types and anatomy could be found, but marked differences between structures were observed, thus suggesting nevertheless a different spatial distribution for I and E populations.
在对猫的半慢性局部麻醉“孤立呼吸中枢”进行延髓和脑桥从颈交界处到呼吸调节系统尾部的地层学探索过程中，记录了呼吸相关单位（RRU）。金属“低阻抗、电容补偿”微电极记录多单位信号，通过计算机对其中的单个放电进行区分和处理；有人认为，使用这些技术，该样本能很好地代表整个单位群体。通过周期触发时间直方图确定与膈神经放电的相位关系。在23000个单位中，28%有明确的呼吸调制。单独检查时，每个RRU都显示出一种稳定的放电模式，对应于各种呼吸类型中的一种，其中大多数以前已经描述过。对RRU放电的时间和空间分布都进行了分析。对随机解剖采样的5000个RRU的峰值放电频率（PFF）的时间分布显示出两个主要群体，其模式分别在吸气（I）或呼气（E）期间观察到。在从I到E以及从E到I的转换处，直方图中出现波谷，这表明在延髓和脑桥直至呼吸调节水平找到跨越相位的RRU的概率较低。这些统计结果与使用与大多数先前研究中类似的先验分类方法获得的结果相同。此外，PFF分布表明，E群体可进一步分为3个子群体，其模式分别落在呼气早期、中期和晚期。比较位于闩前方3毫米处的额 Horsley-Clarke 平面头侧和尾侧两个区域的RRU时间分布，发现在尾侧区域，70%的RRU是I单位，而在头侧延髓和脑桥中，I和E神经元的比例相等。在发现RRU概率较高的解剖结构中分别研究了RRU峰值频率的时间分布。未发现RRU类型与解剖结构之间有明显对应关系，但观察到结构之间存在显著差异，不过这仍表明I和E群体有不同的空间分布。
