挤压压力和脂质性质对脂质囊泡大小及多分散性的影响。
Effect of extrusion pressure and lipid properties on the size and polydispersity of lipid vesicles.
作者信息
Hunter D G, Frisken B J
机构信息
Department of Physics, Simon Fraser University, Burnaby, British Columbia, Canada.
出版信息
Biophys J. 1998 Jun;74(6):2996-3002. doi: 10.1016/S0006-3495(98)78006-3.
The production of vesicles, spherical shells formed from lipid bilayers, is an important aspect of their recent application to drug delivery technologies. One popular production method involves pushing a lipid suspension through cylindrical pores in polycarbonate membranes. However, the actual mechanism by which the polydisperse, multilamellar lipid suspension breaks up into a relatively monodisperse population of vesicles is not well understood. To learn about factors influencing this process, we have characterized vesicles produced under different extrusion parameters and from different lipids. We find that extruded vesicles are only produced above a certain threshold extrusion pressure and have sizes that depend on the extrusion pressure. The minimum pressure appears to be associated with the lysis tension of the lipid bilayer rather than any bending modulus of the system. The flow rate of equal concentration lipid solutions through the pores, after being corrected for the viscosity of water, is independent of lipid properties.
囊泡（由脂质双层形成的球形外壳）的产生是其近期应用于药物递送技术的一个重要方面。一种常用的生产方法是将脂质悬浮液通过聚碳酸酯膜中的圆柱形孔挤出。然而，多分散、多层脂质悬浮液分解成相对单分散的囊泡群体的实际机制尚不清楚。为了了解影响这一过程的因素，我们对在不同挤出参数下和使用不同脂质生产的囊泡进行了表征。我们发现，挤出的囊泡仅在高于某个阈值挤出压力时才会产生，并且其大小取决于挤出压力。最小压力似乎与脂质双层的裂解张力有关，而不是与系统的任何弯曲模量有关。经过水的粘度校正后，等浓度脂质溶液通过孔的流速与脂质性质无关。
Zotero 插件