Tyagi A K, Reddy T L, Venkitasubramanian T A
Can J Microbiol. 1976 Jul;22(7):1054-7. doi: 10.1139/m76-153.
Irradiation with ultraviolet light (360 nm) of cell-free extracts, electron-transport particles, and soluble components from Mycobacterium phlei resulted in the loss of malate oxidation by the flavine adenine dinucleotide pathway both in cell-free extracts and reconstituted systems. Addition of vitamin K1 restored the loss to the extent of 14% and 11% in cell-free extracts and reconstituted systems respectively. Electron-transport particles from M. phlei upon reduction with malate exhibited electron-paramagnetic resonance signals at g = 2.002 and 1.94, characteristic of napthosemiquinone and nonheme iron protein, respectively. Upon irradiating the particles with ultraviolet light (360 nm) these signals were not observed. Particulate flavine-adenine-dinucleotide-dependent malate dehydrogenase (EC 1.1.1.37) of M. phlei assayed by the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide and phenazine methosulfate - 2,6-dichlorophenolindophenol systems, which trap electrons at cytochrome c and at the flavine level respectively, was inhibited by o-phenanthroline. These observations suggest that nonheme iron protein is sensitive to ultraviolet light (360 nm) and participates before or in combination with flavine in the malate (flavine adenine dinucleotide) pathway of M. phlei.
用紫外线（360纳米）照射草分枝杆菌的无细胞提取物、电子传递颗粒和可溶性成分，导致无细胞提取物和重组系统中通过黄素腺嘌呤二核苷酸途径的苹果酸氧化作用丧失。添加维生素K1分别使无细胞提取物和重组系统中的损失恢复了14%和11%。草分枝杆菌的电子传递颗粒在用苹果酸还原后，在g = 2.002和1.94处呈现电子顺磁共振信号，分别为萘半醌和非血红素铁蛋白的特征信号。在用紫外线（360纳米）照射这些颗粒后，未观察到这些信号。用3-（4,5-二甲基噻唑基-2）-2,5-二苯基溴化四氮唑和吩嗪硫酸甲酯 - 2,6-二氯酚靛酚系统测定的草分枝杆菌颗粒性黄素腺嘌呤二核苷酸依赖性苹果酸脱氢酶（EC 1.1.1.37），分别在细胞色素c和黄素水平捕获电子，受到邻菲罗啉的抑制。这些观察结果表明，非血红素铁蛋白对紫外线（360纳米）敏感，并且在草分枝杆菌的苹果酸（黄素腺嘌呤二核苷酸）途径中在黄素之前或与黄素结合参与作用。
