BENZIMAN M, GALANTER Y
J Bacteriol. 1964 Oct;88(4):1010-8. doi: 10.1128/jb.88.4.1010-1018.1964.
Benziman, Moshe (The Hebrew University of Jerusalem, Jerusalem, Israel), and Y. Galanter. Flavine adenine dinucleotide-linked malic dehydrogenase from Acetobacter xylinum. J. Bacteriol. 88:1010-1018. 1964.-The properties of the pyridine nucleotide-nonlinked malic dehydrogenase of Acetobacter xylinum were investigated in the supernatant fluid obtained by high-speed centrifugation of sonic extracts. Ferricyanide, phenazine methosulfate, and to a lesser extent dichlorophenolindophenol were active as oxidants for malate oxidation. After acid ammonium sulfate precipitation, the enzyme lost its malate-oxidizing activity. The enzyme was reactivated by low concentrations of flavine adenine dinucleotide (FAD) but not by flavine mononucleotide (FMN) or riboflavine. Atabrine inhibited the enzyme, and the inhibition was relieved by FAD but not by FMN or riboflavine. Malate-oxidizing activity was inhibited by hematin. The inhibition was prevented by imidazole or globin. o-Phenanthroline, 8-hydroxy quinoline, alpha,alpha'-dipyridyl, and p-chloromercuribenzoate inhibited malate oxidation. Amytal markedly inhibited oxidation of malate in the presence of oxygen, phenazine methosulfate, or dichlorophenolindophenol, but not in the presence of ferricyanide. The results suggest that the malic dehydrogenase of A. xylinum is a FAD enzyme, which contains an ironbinding site essential for its activity. Nonheme iron and sulfhydro groups are possibly involved in enzyme activity. The malic dehydrogenase is functionally linked to the cytochrome chain.
本齐曼，摩西（以色列耶路撒冷希伯来大学），以及Y. 加兰特。木醋杆菌中黄素腺嘌呤二核苷酸连接的苹果酸脱氢酶。《细菌学杂志》88:1010 - 1018。1964年。——对木醋杆菌中吡啶核苷酸非连接的苹果酸脱氢酶的性质进行了研究，该酶存在于经超声提取物高速离心获得的上清液中。铁氰化物、吩嗪硫酸甲酯以及在较小程度上的二氯酚靛酚作为苹果酸氧化的氧化剂具有活性。经酸性硫酸铵沉淀后，该酶失去其苹果酸氧化活性。低浓度的黄素腺嘌呤二核苷酸（FAD）可使该酶重新激活，但黄素单核苷酸（FMN）或核黄素则不能。阿的平抑制该酶，FAD可解除这种抑制，但FMN或核黄素则不能。血红素抑制苹果酸氧化活性。咪唑或珠蛋白可防止这种抑制。邻菲罗啉、8 - 羟基喹啉、α，α'-联吡啶以及对氯汞苯甲酸抑制苹果酸氧化。在有氧、吩嗪硫酸甲酯或二氯酚靛酚存在的情况下，阿米妥显著抑制苹果酸氧化，但在铁氰化物存在时则不然。结果表明，木醋杆菌的苹果酸脱氢酶是一种FAD酶，其含有对其活性至关重要的铁结合位点。非血红素铁和巯基可能参与酶活性。苹果酸脱氢酶在功能上与细胞色素链相联系。
