Ran结合蛋白家族的成员Yrb2p参与核蛋白输出。
A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export.
作者信息
Taura T, Krebber H, Silver P A
机构信息
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School and The Dana-Farber Cancer Institute, 44 Binney Street, Boston, MA 02115, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7427-32. doi: 10.1073/pnas.95.13.7427.
Abstract
Yeast cells mutated in YRB2, which encodes a nuclear protein with similarity to other Ran-binding proteins, fail to export nuclear export signal (NES)-containing proteins including HIV Rev out of the nucleus. Unlike Xpo1p/Crm1p/exportin, an NES receptor, Yrb2p does not shuttle between the nucleus and the cytoplasm but instead remains inside the nucleus. However, by both biochemical and genetic criteria, Yrb2p interacts with Xpo1p and not with other members of the importin/karyopherin beta superfamily. Moreover, the Yrb2p region containing nucleoporin-like FG repeats is important for NES-mediated protein export. Taken together, these data suggest that Yrb2p acts inside the nucleus to mediate the action of Xpo1p in at least one of several nuclear export pathways.
摘要
YRB2发生突变的酵母细胞无法将包括HIV Rev在内的含有核输出信号（NES）的蛋白质输出细胞核，YRB2编码一种与其他Ran结合蛋白相似的核蛋白。与NES受体Xpo1p/Crm1p/输出蛋白不同，Yrb2p不会在细胞核和细胞质之间穿梭，而是留在细胞核内。然而，根据生化和遗传学标准，Yrb2p与Xpo1p相互作用，而不与输入蛋白/核转运蛋白β超家族的其他成员相互作用。此外，含有核孔蛋白样FG重复序列的Yrb2p区域对NES介导的蛋白质输出很重要。综上所述，这些数据表明Yrb2p在细胞核内发挥作用，至少在几种核输出途径之一中介导Xpo1p的作用。
相似文献
1
A member of the Ran-binding protein family, Yrb2p, is involved in nuclear protein export.Ran结合蛋白家族的成员Yrb2p参与核蛋白输出。
Proc Natl Acad Sci U S A. 1998 Jun 23;95(13):7427-32. doi: 10.1073/pnas.95.13.7427.
2
Structural insights into how Yrb2p accelerates the assembly of the Xpo1p nuclear export complex.关于Yrb2p如何加速Xpo1p核输出复合物组装的结构见解。
Cell Rep. 2014 Nov 6;9(3):983-95. doi: 10.1016/j.celrep.2014.09.052. Epub 2014 Oct 30.
3
The nuclear export receptor Xpo1p forms distinct complexes with NES transport substrates and the yeast Ran binding protein 1 (Yrb1p).核输出受体Xpo1p与核输出信号（NES）转运底物和酵母Ran结合蛋白1（Yrb1p）形成不同的复合物。
Mol Biol Cell. 2001 Mar;12(3):539-49. doi: 10.1091/mbc.12.3.539.
4
Exportin 1 (Crm1p) is an essential nuclear export factor.输出蛋白1（Crm1p）是一种重要的核输出因子。
Cell. 1997 Sep 19;90(6):1041-50. doi: 10.1016/s0092-8674(00)80370-0.
5
Disruption of the YRB2 gene retards nuclear protein export, causing a profound mitotic delay, and can be rescued by overexpression of XPO1/CRM1.YRB2基因的破坏会延缓核蛋白输出，导致严重的有丝分裂延迟，并且可以通过过表达XPO1/CRM1来挽救。
J Biochem. 1999 Mar;125(3):574-85. doi: 10.1093/oxfordjournals.jbchem.a022323.
6
The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export.输入蛋白β家族成员Crm1p在核输出过程中介导了Rev与核孔复合体之间的相互作用。
Curr Biol. 1997 Oct 1;7(10):767-75. doi: 10.1016/s0960-9822(06)00335-6.
7
The NES-Crm1p export pathway is not a major mRNA export route in Saccharomyces cerevisiae.NES-Crm1p 输出途径并非酿酒酵母中主要的 mRNA 输出途径。
EMBO J. 1999 Jul 1;18(13):3746-56. doi: 10.1093/emboj/18.13.3746.
8
Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue.Yrb2p是一种与酵母Rcc1同源物Prp20p相互作用的核蛋白。
J Biol Chem. 1997 Dec 12;272(50):31877-84. doi: 10.1074/jbc.272.50.31877.
9
Nuclear export of yeast signal recognition particle lacking Srp54p by the Xpo1p/Crm1p NES-dependent pathway.通过Xpo1p/Crm1p的NES依赖性途径对缺乏Srp54p的酵母信号识别颗粒进行核输出。
Curr Biol. 2000 Oct 19;10(20):1256-64. doi: 10.1016/s0960-9822(00)00743-0.
10
Interactions between a nuclear transporter and a subset of nuclear pore complex proteins depend on Ran GTPase.一种核转运蛋白与核孔复合体蛋白的一个子集之间的相互作用取决于Ran GTP酶。
Mol Cell Biol. 1999 Feb;19(2):1547-57. doi: 10.1128/MCB.19.2.1547.
引用本文的文献
1
Dynamics of nuclear export of pre-ribosomal subunits revealed by high-speed single-molecule microscopy in live cells.活细胞中高速单分子显微镜揭示的核糖体前体亚基核输出动力学
iScience. 2023 Jul 21;26(8):107445. doi: 10.1016/j.isci.2023.107445. eCollection 2023 Aug 18.
2
Capturing the Asc1p/eceptor for ctivated inase (RACK1) Microenvironment at the Head Region of the 40S Ribosome with Quantitative BioID in Yeast.利用酵母中定量生物 ID 技术捕获 40S 核糖体头部区域的 Asc1p/eceptor for ctivated inase (RACK1) 微环境。
Mol Cell Proteomics. 2017 Dec;16(12):2199-2218. doi: 10.1074/mcp.M116.066654. Epub 2017 Oct 5.
3
mRNA quality control is bypassed for immediate export of stress-responsive transcripts.mRNA质量控制被绕过，以便应激反应性转录本立即输出。
Nature. 2016 Dec 22;540(7634):593-596. doi: 10.1038/nature20572. Epub 2016 Dec 12.
4
Assembly and nuclear export of pre-ribosomal particles in budding yeast.芽殖酵母中前核糖体颗粒的组装与核输出
Chromosoma. 2014 Aug;123(4):327-44. doi: 10.1007/s00412-014-0463-z. Epub 2014 May 11.
5
Proteasome-mediated processing of Def1, a critical step in the cellular response to transcription stress.蛋白酶体介导的 Def1 加工，是细胞对应转录应激的关键步骤。
Cell. 2013 Aug 29;154(5):983-995. doi: 10.1016/j.cell.2013.07.028.
6
The intrinsic GTPase activity of the Gtr1 protein from Saccharomyces cerevisiae.酿酒酵母 Gtr1 蛋白的内在 GTPase 活性。
BMC Biochem. 2012 Jun 24;13:11. doi: 10.1186/1471-2091-13-11.
7
Ran-dependent nuclear export mediators: a structural perspective.依赖 Ran 的核输出介体：结构视角。
EMBO J. 2011 Aug 31;30(17):3457-74. doi: 10.1038/emboj.2011.287.
8
Human T lymphotropic virus type 1 regulatory and accessory gene transcript expression and export are not rex dependent.人类嗜T淋巴细胞病毒1型调节基因和辅助基因转录本的表达及输出不依赖于rex。
AIDS Res Hum Retroviruses. 2012 Apr;28(4):405-10. doi: 10.1089/aid.2011.0130. Epub 2011 Aug 5.
9
The Ccr4-Not complex interacts with the mRNA export machinery.Ccr4-Not 复合物与 mRNA 输出机制相互作用。
PLoS One. 2011 Mar 28;6(3):e18302. doi: 10.1371/journal.pone.0018302.
10
An allosteric mechanism to displace nuclear export cargo from CRM1 and RanGTP by RanBP1.RanBP1 通过变构机制从 CRM1 和 RanGTP 上置换核输出货物。
EMBO J. 2010 Jun 16;29(12):2002-13. doi: 10.1038/emboj.2010.89. Epub 2010 May 18.
本文引用的文献
1
A distinct and parallel pathway for the nuclear import of an mRNA-binding protein.一条用于mRNA结合蛋白核输入的独特且平行的途径。
J Cell Biol. 1997 Dec 29;139(7):1645-53. doi: 10.1083/jcb.139.7.1645.
2
Yrb2p is a nuclear protein that interacts with Prp20p, a yeast Rcc1 homologue.Yrb2p是一种与酵母Rcc1同源物Prp20p相互作用的核蛋白。
J Biol Chem. 1997 Dec 12;272(50):31877-84. doi: 10.1074/jbc.272.50.31877.
3
CRM1 is responsible for intracellular transport mediated by the nuclear export signal.CRM1负责由核输出信号介导的细胞内运输。
Nature. 1997 Nov 20;390(6657):308-11. doi: 10.1038/36894.
4
The importin-beta family member Crm1p bridges the interaction between Rev and the nuclear pore complex during nuclear export.输入蛋白β家族成员Crm1p在核输出过程中介导了Rev与核孔复合体之间的相互作用。
Curr Biol. 1997 Oct 1;7(10):767-75. doi: 10.1016/s0960-9822(06)00335-6.
5
Export of importin alpha from the nucleus is mediated by a specific nuclear transport factor.输入蛋白α从细胞核的输出由一种特定的核转运因子介导。
Cell. 1997 Sep 19;90(6):1061-71. doi: 10.1016/s0092-8674(00)80372-4.
6
CRM1 is an export receptor for leucine-rich nuclear export signals.CRM1是富含亮氨酸的核输出信号的输出受体。
Cell. 1997 Sep 19;90(6):1051-60. doi: 10.1016/s0092-8674(00)80371-2.
7
Exportin 1 (Crm1p) is an essential nuclear export factor.输出蛋白1（Crm1p）是一种重要的核输出因子。
Cell. 1997 Sep 19;90(6):1041-50. doi: 10.1016/s0092-8674(00)80370-0.
8
Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.Yrb4p，一种参与核糖体蛋白L25导入细胞核过程的酵母Ran-GTP结合蛋白。
EMBO J. 1997 Oct 15;16(20):6237-49. doi: 10.1093/emboj/16.20.6237.
9
Evidence for a role of CRM1 in signal-mediated nuclear protein export.CRM1在信号介导的核蛋白输出中作用的证据。
Science. 1997 Oct 3;278(5335):141-4. doi: 10.1126/science.278.5335.141.
10
Importin/karyopherin protein family members required for mRNA export from the nucleus.mRNA从细胞核输出所需的输入蛋白/核转运蛋白家族成员。
Proc Natl Acad Sci U S A. 1997 Aug 5;94(16):8590-5. doi: 10.1073/pnas.94.16.8590.
Zotero 插件