Wold W S, Suzuki I
Can J Microbiol. 1976 Aug;22(8):1083-92. doi: 10.1139/m76-159.
The citric acid fermentation by Aspergillus niger is divided into two consecutive phases, a growth phase when the cells proliferate but do not accumulate citrate, followed by an accumulating phase when they produce citrate but do not proliferate, or else do so at a much reduced rate. When studied in a low sucrose (0.4-0.8%) mpinimal salts medium the growth-accumulation alternative was controlled by the concentration of zinc: high zinc (about 1-2 muM) maintained growth phase, while at low zinc (below 1 muM) growth became limited by zinc deficiency and the cultures passed into accumulating phase. Addition of zinc to accumulating cultures resulted in their reversion to growth phase. Iron, manganese, calcium at concentrations as high as 5-10 muM had no influence on either growth or citrate accumulation. These results suggest that zinc plays a role in the regulation of growth and citric acid accumulation.
黑曲霉的柠檬酸发酵分为两个连续阶段，一个是生长阶段，细胞在此阶段增殖但不积累柠檬酸，随后是积累阶段，细胞在此阶段产生柠檬酸但不增殖，或者增殖速度大幅降低。在低蔗糖（0.4 - 0.8%）的基本盐培养基中研究时，生长 - 积累的转变由锌浓度控制：高锌（约1 - 2μM）维持生长阶段，而低锌（低于1μM）时生长受锌缺乏限制，培养物进入积累阶段。向处于积累阶段的培养物中添加锌会使其恢复到生长阶段。铁、锰、钙浓度高达5 - 10μM时对生长或柠檬酸积累均无影响。这些结果表明锌在生长和柠檬酸积累的调节中起作用。
