Biocca S, Ruberti F, Tafani M, Pierandrei-Amaldi P, Cattaneo A
Institute of Neurobiology, CNR, Roma, Italy.
Biotechnology (N Y). 1995 Oct;13(10):1110-5. doi: 10.1038/nbt1095-1110.
In this paper we have engineered the targeting of ScFv fragments to mitochondria and demonstrated that this can occur efficiently. This extends the range of subcellular compartments where antibody domains can be targeted in order to interfere with the action of the corresponding antigen. Moreover, we have compared the redox state of ScFv fragments targeted to the secretory compartment, the cytosol and the mitochondria, and demonstrated that cysteine residues in ScFv targeted to the secretory compartments and to the mitochondria are oxidized. On the contrary, cytosolic antibody domains are expressed in a reduced state, which is probably the reason for their lower expression levels. These pitfalls, however, do not prevent their successful utilization for intracellular immunization.
在本文中，我们设计了将单链抗体片段靶向线粒体的方法，并证明这一过程可以高效发生。这扩展了抗体结构域可靶向的亚细胞区室范围，以便干扰相应抗原的作用。此外，我们比较了靶向分泌区室、细胞质和线粒体的单链抗体片段的氧化还原状态，证明靶向分泌区室和线粒体的单链抗体中的半胱氨酸残基被氧化。相反，细胞质中的抗体结构域以还原状态表达，这可能是其表达水平较低的原因。然而，这些问题并不妨碍它们成功用于细胞内免疫。
