一种自动化顶空-气相色谱法在大鼠组织中二甲苯和乙苯分配系数测定中的应用。

Application of an automated HS-GC method in partition coefficient determination for xylenes and ethylbenzene in rat tissues.

作者信息

Kumarathasan P, Otson R, Chu I

出版信息

Chemosphere. 1998 Jul;37(1):159-78. doi: 10.1016/s0045-6535(98)00031-9.

Abstract

An automated static head space-gas chromatography method was used in the determination of partition coefficients (Kd) for the xylene isomers and ethylbenzene in blood, brain, muscle, kidney, liver and fat of Sprague Dawley rats. Since homogenization resulted in the potential loss of analytes from tissue samples, unhomogenized samples were used. With a few exceptions, tissue:air Kd values were independent of the concentrations of the analytes, singly or as a mixture. The tissue:blood Kd values were determined. For each tissue and analyte, the value obtained for each analyte concentration was within +/- 10% of the mean value calculated for the entire concentration range.

摘要

采用自动静态顶空-气相色谱法测定了斯普拉格-道利大鼠血液、脑、肌肉、肾脏、肝脏和脂肪中二甲苯异构体和乙苯的分配系数（Kd）。由于匀浆会导致组织样品中分析物的潜在损失，因此使用了未匀浆的样品。除了少数例外情况，组织与空气的Kd值与分析物的浓度无关，无论是单独存在还是混合存在。测定了组织与血液的Kd值。对于每种组织和分析物，每个分析物浓度所获得的值在整个浓度范围内计算出的平均值的±10%以内。