Tsuboi T, Imada T
Laboratory of Microbiological Diagnosis, Department of Systematic Diagnosis, National Institute of Animal Health, Tsukuba, Ibaraki, Japan.
J Vet Med Sci. 1998 May;60(5):569-72. doi: 10.1292/jvms.60.569.
Bovine follicle fluid and oocytes surrounded by follicular epithelial (FE) cells were collected from ovaries of two heifers persistently infected with bovine viral diarrhea virus (BVDV). BVDV was present in the follicle fluid at a higher titer than in serum. The oocytes were matured in vitro under culture conditions of 39 degrees C in humidified air containing 5% CO2. In vitro fertilization was performed after 24 hr in culture (the day of insemination was defined as day 1), and culture was continued through day 10. BVDV was present in the culture medium at titers of 10(2.25) to 10(3.25) TC(I)D50/0.1 ml. The virus was also detected in FE cells collected on day 10. Viral antigen was demonstrated in the cytoplasm of FE cells by the indirect immunofluorescence technique. However, no BVDV was detected in the embryos on day 10. These findings suggested that the oocytes or embryos were unlikely to be infected with BVDV, but that the FE cells were infected with BVDV and supported virus replication in cattle persistently infected with BVDV.
从两头持续感染牛病毒性腹泻病毒（BVDV）的小母牛卵巢中采集牛卵泡液和被卵泡上皮（FE）细胞包围的卵母细胞。卵泡液中BVDV的滴度高于血清。卵母细胞在39℃、含5%二氧化碳的湿润空气中的培养条件下进行体外成熟。培养24小时后进行体外受精（授精日定义为第1天），并持续培养至第10天。培养基中BVDV的滴度为10(2.25)至10(3.25) TC(I)D50/0.1 ml。在第10天收集的FE细胞中也检测到该病毒。通过间接免疫荧光技术在FE细胞的细胞质中证实了病毒抗原。然而，在第10天的胚胎中未检测到BVDV。这些发现表明，卵母细胞或胚胎不太可能感染BVDV，但FE细胞感染了BVDV并支持持续感染BVDV的牛体内的病毒复制。
