Sakai H, Nonaka N, Yagi K, Oku Y, Kamiya M
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
J Vet Med Sci. 1998 May;60(5):639-41. doi: 10.1292/jvms.60.639.
Detection of Echinococcus coproantigen using sandwich enzyme-linked immunosorbent assay (sELISA) was performed on fecal samples of red foxes in Hokkaido, Japan. Fecal samples were collected around fox dens in 1990 and 1992. The antibodies used for sELISA recognize heat-resistant antigens, thus all fecal samples were heated to render it safe for handling before examination. Detection of taeniid egg in fox feces collected was considered as an indication of E. multilocularis infection. In fecal samples collected in 1990 and 1992, coproantigen positive results out of taeniid-egg positive cases were 38/40 (95.0%) and 95/97 (97.9%), respectively. In addition, coproantigen was detected regardless of fecal condition when collected from the field, suggesting that the antigens detected by this method are quite stable. These results suggest that detection of coproantigen is useful for field surveys of foxes naturally infected with E. multilocularis.
利用夹心酶联免疫吸附测定法（sELISA）对日本北海道赤狐的粪便样本进行细粒棘球绦虫粪抗原检测。粪便样本于1990年和1992年在狐狸洞穴周围采集。用于sELISA的抗体识别耐热抗原，因此所有粪便样本在检测前均经过加热处理以确保处理安全。检测所采集狐狸粪便中的带绦虫卵被视为多房棘球绦虫感染的指标。在1990年和1992年采集的粪便样本中，带绦虫卵阳性病例中的粪抗原阳性结果分别为38/40（95.0%）和95/97（97.9%）。此外，从野外采集的粪便无论其状态如何均检测到粪抗原，这表明该方法检测到的抗原相当稳定。这些结果表明，粪抗原检测对于自然感染多房棘球绦虫的狐狸的野外调查很有用。
