Machnicka B, Dziemian E, Rocki B, Kołodziej-Sobocińska M
Institute of Parasitology, Polish Academy of Sciences, Twarda str 51/55, 00-818, Warsaw, Poland.
Parasitol Res. 2003 Dec;91(6):491-6. doi: 10.1007/s00436-003-0994-3. Epub 2003 Oct 7.
Faecal samples deriving from 391 animals belonging to nine species (polecats, badgers, martens, weasels, rats, dogs, cats, red foxes, raccoon-dogs) were examined by capture ELISA for the presence of the Echinococcus multilocularis coproantigen. The main claim of our studies is the reliable detection of E. multilocularis coproantigens, mainly in the faeces of foxes, dogs and cats. For the first time in coproantigen detection we used a "double-sandwich" ELISA. The main advantage of this method is the higher specificity and better differentiation of positive and negative faecal samples, in comparison with sandwich ELISA. The overall specificity of double-sandwich ELISA was 95.1% with only 16 of 327 E. multilocularis-free animals giving false-positive results. The E. multilocularis coproantigen was detected by double-sandwich ELISA in 37.5% of examined red foxes and in 8.0% of examined raccoon-dogs, compared with a prevalence of just 29.8% in red foxes and 8.0% in raccoon-dogs, as determined by parasitological techniques.
采用捕获ELISA法检测了来自9个物种（鸡貂、獾、貂、黄鼬、大鼠、狗、猫、赤狐、貉）的391只动物的粪便样本，以检测多房棘球绦虫粪抗原的存在。我们研究的主要成果是可靠地检测到了多房棘球绦虫粪抗原，主要存在于狐狸、狗和猫的粪便中。在粪抗原检测中，我们首次使用了“双夹心”ELISA法。与夹心ELISA相比，该方法的主要优点是具有更高的特异性，能更好地区分粪便样本的阳性和阴性。双夹心ELISA的总体特异性为95.1%，在327只未感染多房棘球绦虫的动物中，只有16只给出了假阳性结果。通过双夹心ELISA法，在37.5%的被检测赤狐和8.0%的被检测貉中检测到了多房棘球绦虫粪抗原，而通过寄生虫学技术测定，赤狐的患病率仅为29.8%，貉的患病率为8.0%。
