通过原位杂交和原位PCR检测非肿瘤性扁桃体上皮细胞和淋巴细胞中的爱泼斯坦-巴尔病毒感染。

Detection of Epstein-Barr virus infection in the epithelial cells and lymphocytes of non-neoplastic tonsils by in situ hybridization and in situ PCR.

作者信息

Kobayashi R, Takeuchi H, Sasaki M, Hasegawa M, Hirai K

机构信息

Department of Forensic Medicine, Juntendo University School of Medicine, Tokyo, Japan.

出版信息

Arch Virol. 1998;143(4):803-13. doi: 10.1007/s007050050332.

DOI:10.1007/s007050050332

PMID:9638150

Abstract

Non-neoplastic tonsils were analyzed for detection of Epstein-Barr virus (EBV)-positive cells by in situ hybridization and in situ PCR. EBV-encoded small nuclear RNA 1(EBER1)-positive cells were found in 28.2% of the tonsils and were evenly localized in the extrafollicular area and within germinal centers. Latent membrane protein 1 (LMP1)-positive cells were also dispersed in the extrafollicular and germinal center. Using in situ DNA-DNA hybridization, the EBV-positive signals were observed in the upper epithelial cell layers of the tonsils. In addition, in situ PCR detected EBV DNA-positive cells in the lower epithelial cell layers and lymphoid cells.

摘要

通过原位杂交和原位PCR分析非肿瘤性扁桃体，以检测爱泼斯坦-巴尔病毒（EBV）阳性细胞。在28.2%的扁桃体中发现了EBV编码的小核RNA 1（EBER1）阳性细胞，这些细胞均匀分布在滤泡外区域和生发中心内。潜伏膜蛋白1（LMP1）阳性细胞也分散在滤泡外和生发中心。使用原位DNA-DNA杂交技术，在扁桃体的上层上皮细胞层中观察到EBV阳性信号。此外，原位PCR在下层上皮细胞层和淋巴细胞中检测到EBV DNA阳性细胞。