Adrian K, Mellgren K, Skogby M, Friberg L G, Mellgren G, Wadenvik H
Department of Paediatrics, Sahlgrenska University Hospital/Ostra, Göteborg, Sweden.
Perfusion. 1998 May;13(3):187-91. doi: 10.1177/026765919801300306.
The objective of this study was to evaluate the effect of albumin priming on platelet consumption and activation during long-term perfusion. Two identical in vitro extracorporeal membrane oxygenation circuits were used; one was primed with Ringer's solution containing human serum albumin, the other with Ringer's solution only. Fresh heparinized human blood was pooled, divided between the two systems and circulated for 24 h at 37 degrees C. Platelet count, plasma concentration of betathromboglobulin (BTG), platelet membrane density of glycoprotein (GP) Ib and of GPIIb/IIIa were assayed before the start and at 0.5, 1, 3, 12 and 24 h of perfusion. In total, seven experiments were performed. We found that during the first hour of perfusion, slightly higher platelet counts (p = 0.058) and lower BTG values (p = 0.0005) were observed in the circuits primed with albumin, compared to the control circuits. No statistically significant differences were observed for the platelet membrane expression of GPIb and GPIIb/IIIa. We conclude that albumin priming appears to transiently prevent platelet consumption and activation during long-term perfusion.
本研究的目的是评估白蛋白预充对长期灌注期间血小板消耗和活化的影响。使用了两个相同的体外膜肺氧合回路；一个用含人血清白蛋白的林格氏液预充，另一个仅用林格氏液预充。采集新鲜肝素化的人血，分置于两个系统中，并在37℃下循环24小时。在灌注开始前以及灌注0.5、1、3、12和24小时时，检测血小板计数、β-血小板球蛋白（BTG）的血浆浓度、糖蛋白（GP）Ib和GPIIb/IIIa的血小板膜密度。总共进行了七项实验。我们发现，在灌注的第一个小时内，与对照回路相比，用白蛋白预充的回路中观察到血小板计数略高（p = 0.058），BTG值略低（p = 0.0005）。在GPIb和GPIIb/IIIa的血小板膜表达方面未观察到统计学上的显著差异。我们得出结论，白蛋白预充似乎在长期灌注期间可暂时防止血小板的消耗和活化。
