Guida G, Maida I, Gallone A, Boffoli D, Cicero R
Istituto di Biologia Generale, Faculty of Medicine and Surgery, University of Bari, Italy.
In Vitro Cell Dev Biol Anim. 1998 May;34(5):393-400. doi: 10.1007/s11626-998-0021-1.
A study of the liver pigment cells of Rana esculenta L. has been performed on both liver in toto and cells in culture. Ultrastructural and cytochemical analyses showed a close relationship between this visceral pigment cell system and the cells of hepatic macrophage lineage. Like the latter, the liver pigment cells present phagocytic activity, in the sinusoids and in vitro, and give a positive response to tests for peroxidase and lipase. The liver pigment cells are isolated, together with the Kupffer cells, from the sinusoidal cell fraction of the liver. In culture, they maintain their melanogenetic ability, demonstrated by the presence of dopaoxidase activity in the soluble, membranous, and melanosome fractions. Analysis of the cultures showed that as culture time increased, so did melanosome dopaoxidase activity, the number of pigmented fields, and the level of pigmentation of the cells. The values of dopaoxidase activity of the pigment cells in culture show the same seasonal oscillations as the system in toto, indicating that the cells maintain an internal clock, at least in the first 72 h of culture. There is evidence that the pigment cells are macrophages which can express a melanogenetic function. Our results and other experimental data provide a basis for hypothesizing that the pigment cells in Rana esculenta L. liver may derive from, or have a common origin with, the Kupffer cells.
对食用蛙肝脏色素细胞的研究是在整个肝脏和培养细胞上进行的。超微结构和细胞化学分析表明，这种内脏色素细胞系统与肝巨噬细胞系的细胞之间存在密切关系。与后者一样，肝脏色素细胞在肝血窦内和体外均呈现吞噬活性，并且对过氧化物酶和脂肪酶检测呈阳性反应。肝脏色素细胞与库普弗细胞一起，从肝脏的血窦细胞组分中分离出来。在培养过程中，它们保持其黑色素生成能力，这通过在可溶性、膜性和黑素小体组分中存在多巴氧化酶活性得以证明。对培养物的分析表明，随着培养时间的增加，黑素小体多巴氧化酶活性、色素沉着区域的数量以及细胞的色素沉着水平也随之增加。培养物中色素细胞的多巴氧化酶活性值与整个系统呈现相同的季节性振荡，这表明细胞至少在培养的前72小时内保持着内部时钟。有证据表明色素细胞是能够表达黑色素生成功能的巨噬细胞。我们的结果和其他实验数据为推测食用蛙肝脏中的色素细胞可能源自库普弗细胞或与库普弗细胞有共同起源提供了依据。
