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来自乳酸克鲁维酵母的HIS4基因。

The HIS4 gene from the yeast Kluyveromyces lactis.

作者信息

Freire-Picos M A, Hampsey M, Cerdán M E

机构信息

Departamento de Biologia Celular y Molecular, Facultad de Ciencias, Universidad de La Coruña, Spain.

出版信息

Yeast. 1998 May;14(7):687-91. doi: 10.1002/(SICI)1097-0061(199805)14:7<687::AID-YEA261>3.0.CO;2-4.

DOI:10.1002/(SICI)1097-0061(199805)14:7<687::AID-YEA261>3.0.CO;2-4
PMID:9639316
Abstract

The Kluyveromyces lactis HIS4 gene was cloned by complementation of a Saccharomyces cerevisiae his4 mutant. Sequence analysis revealed a 2388 bp open reading frame encoding a single polypeptide predicted to encompass three distinct enzymatic activities (phosphoribosyl-AMP cyclohydrolase, phosphoribosyl-ATP pyrophosphohydrolase and histidinol dehydrogenase). This structural organization is strikingly similar to that of the His4 proteins from S. cerevisiae and Pichia pastoris. Transcript analysis detected a single mRNA species of 2.5 kb.

摘要

通过互补酿酒酵母his4突变体克隆了乳酸克鲁维酵母HIS4基因。序列分析揭示了一个2388 bp的开放阅读框，其编码一种预测包含三种不同酶活性（磷酸核糖-AMP环水解酶、磷酸核糖-ATP焦磷酸水解酶和组氨醇脱氢酶）的单一多肽。这种结构组织与酿酒酵母和巴斯德毕赤酵母的His4蛋白惊人地相似。转录分析检测到一种2.5 kb的单一mRNA种类。

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