[孕酮对体外培养的胎鼠颅骨成骨细胞增殖与分化的影响]
[Effects of progesterone on proliferation and differentiation of fetal rat calvarial osteoblasts in vitro].
作者信息
Chen L, Schøller J, Foged N T
机构信息
Union Hospital, Tongji Medical University, Wuhan.
出版信息
Zhonghua Fu Chan Ke Za Zhi. 1997 Sep;32(9):538-40.
OBJECTIVES
To investigate the influence of progesterone on proliferation and differentiation of osteoblast at the levels of gene expression and cell functions.
METHODS
Fetal rat calvarial osteoblasts were cultured in vitro in the presence of (10(-9) mol/L-10(-6) mol/L) progesterone. Cell proliferation, alkaline phosphalase (ALP) activity, osteocalcin mRNA expression and osteocalcin secretion in the medium and bone nodule formation were analyzed.
RESULTS
Progesterone did not influence cell proliferation; Progesterone enhanced the ALP activity in rat osteoblasts; Progesterone stimulated osteocalcin mRNA expression in a dose-dependent manner and increased the amount of osteocalcin in the culture medium; Progesterone increased both number and area of bone nodule formation.
CONCLUSION
Progesterone has a multi-stimulating effect on the differentiation of fetal rat calvarial osteoblast, hut no effect on cell proliferation.
目的
在基因表达和细胞功能水平上研究孕酮对成骨细胞增殖和分化的影响。
方法
将胎鼠颅骨成骨细胞在含有（10⁻⁹mol/L - 10⁻⁶mol/L）孕酮的条件下进行体外培养。分析细胞增殖、碱性磷酸酶（ALP）活性、骨钙素mRNA表达、培养基中骨钙素分泌以及骨结节形成情况。
结果
孕酮不影响细胞增殖；孕酮增强了大鼠成骨细胞的ALP活性；孕酮以剂量依赖方式刺激骨钙素mRNA表达，并增加培养基中骨钙素的量；孕酮增加了骨结节形成的数量和面积。
结论
孕酮对胎鼠颅骨成骨细胞的分化具有多重刺激作用，但对细胞增殖无影响。
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