Xu Ling, Liang Xing, Huang Jiao, Dong Qiang, Xia Lu, Hu Jian, Zhang Qing-hong, Li Xiao-yu
Department of Prosthodontics, West China Stomatological Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 May;37(3):445-8.
To investigate the effect of osteogenic growth peptide(OGP) to the proliferation and differentiation of osteoblast-like cells in vitro.
Calvarial osteoblast-like cells of rat bones were cultured individually in medium contained various concentrations (10(-11) mol/L-10(-7) mol/L) of OGP; and the cell proliferation was checked by MTT method, alkaline phosphatase activity was detected by ELISA and level of Cbfal mRNA was amplified with RT-PCR.
In vitro, the OGP put in culture medium resulted in an increase of cell proliferation (P < 0.05), ALP activity (P < 0.05) and level of mRNA for Cbfal (P < 0.05) respectively. All used OGP concentrations (10(-11) mol/L-10(-7) mol/L) were effective but the maximum efficiency was obtained with 10(-8) mol/L OGP.
Our results demonstrate that OGP stimulates proliferation and sequential differentiation in vitro of calvarial osteoblast-like cells of rat bones.
研究成骨生长肽（OGP）对体外培养的大鼠颅骨成骨样细胞增殖和分化的影响。
将大鼠颅骨成骨样细胞分别培养于含有不同浓度（10⁻¹¹mol/L - 10⁻⁷mol/L）OGP的培养基中；采用MTT法检测细胞增殖情况，用ELISA法检测碱性磷酸酶活性，通过RT-PCR扩增Cbfal mRNA水平。
在体外，培养基中加入OGP分别导致细胞增殖增加（P < 0.05）、碱性磷酸酶活性增加（P < 0.05）以及Cbfal mRNA水平增加（P < 0.05）。所有使用的OGP浓度（10⁻¹¹mol/L - 10⁻⁷mol/L）均有效，但10⁻⁸mol/L的OGP效果最佳。
我们的结果表明，OGP可刺激大鼠颅骨成骨样细胞在体外的增殖和连续分化。
