Sun H, Liu W, Shao J, Xu H, Xiao K, Sheng G
Department of Internal Medicine, Tongji Hospital, Tongji medical University, Wuhan.
J Tongji Med Univ. 1997;17(1):18-20, 24. doi: 10.1007/BF02887995.
The immunoregulating effect of Interleukin-1-receptor antagonist (IL-1ra) in lupus-like NZB/W F1 mice was investigated to find possible approach to prevent lupus nephritis. 12 female NZB/W F1 mice of 13 weeks were randomly divided into 2 groups. Each mouse in the treated group was intraperitoneally injected with IL-1ra once every 2 weeks for 3 times at the dosage of 100 micrograms each time, while the control group was given injection of 0.1 ml normal saline. All the mice were killed at the age of 9 months and the immunologic function was examined. Results showed that this dosage could not completely prevent the development of lupus nephritis, but the renal damage was alleviated and the urine protein was decreased. Moreover, it could improve the immunofunction by significantly reducing the levels of serum IL-1 and obviously increase the activities of NK cells and IL-2 induced by ConA in mononuclear cells of spleen. There was no significant difference in the levels of serum IL-6 and TNF-alpha between the treated group and control group. It is concluded that IL-1ra has certain regulatory effect on the immunologic function of lupus-like NZB/W F1 mice.
研究白细胞介素-1受体拮抗剂（IL-1ra）对狼疮样NZB/W F1小鼠的免疫调节作用，以寻找预防狼疮性肾炎的可能方法。将12只13周龄的雌性NZB/W F1小鼠随机分为2组。治疗组每只小鼠每2周腹腔注射一次IL-1ra，每次剂量为100微克，共注射3次，而对照组注射0.1毫升生理盐水。所有小鼠在9月龄时处死并检查免疫功能。结果表明，该剂量不能完全预防狼疮性肾炎的发生，但可减轻肾脏损伤并降低尿蛋白。此外，它可通过显著降低血清IL-1水平来改善免疫功能，并明显增加脾脏单个核细胞中由刀豆蛋白A诱导的NK细胞活性和IL-2活性。治疗组和对照组之间血清IL-6和TNF-α水平无显著差异。结论是，IL-1ra对狼疮样NZB/W F1小鼠的免疫功能有一定的调节作用。
