Chen X, He S, Long L, Huan L, Xue Y
Institute of Microbiology, Academia Sinica, Beijing.
Wei Sheng Wu Xue Bao. 1996 Aug;36(4):269-75.
Nisin from Lactococcus lactis subsp. lactis AL2 was extracted with n-propanol from NaCl-saturated culture and purified by ion-exchange chromotography on CM-Sephadex C-25. Nisin was purified 1.63 fold with a yield of 41.7%. The molecular weight of nisin was determined by SDS-PAGE to be about 3500. Nisin activity was stable at low pH and sensitive to digestion by a-chymotrypsin. Nisin is capable of inhibiting a broad range of gram-positive bacteria. In contrast, the gram-negative bacteria, yeasts, molds and Nip+ L. lactis subsp. lactis ATCC11454 were not inhibited.
从乳酸乳球菌乳酸亚种AL2中提取的乳链菌肽，用正丙醇从饱和氯化钠培养物中提取，并通过CM-葡聚糖凝胶C-25离子交换色谱法纯化。乳链菌肽纯化了1.63倍，产率为41.7%。通过SDS-PAGE测定乳链菌肽的分子量约为3500。乳链菌肽活性在低pH值下稳定，对α-胰凝乳蛋白酶消化敏感。乳链菌肽能够抑制多种革兰氏阳性菌。相比之下，革兰氏阴性菌、酵母、霉菌和乳酸乳球菌乳酸亚种ATCC11454未受到抑制。
