Dai S, Gao M, Li X, Li R
Wuhan Institute of Virology, Academia Sinica, Wuhan.
Wei Sheng Wu Xue Bao. 1996 Aug;36(4):295-302.
221 isolates of Bacillus thuringiensis were isolated in 1491 soil samples from North and South of China. H-serotypes and larvicidal characters of all Bt isolates have been identified. The rate of Bt-harbouring soil sample and the rate of Bt isolates in Northeast and Neimeng were in 12.6% and 17.2% respectively. Predominant serotypes were H4, H10, H3, H13, H5 and H29. The most fertile Bt-harbouring area was the Heilongjiang Province with rate of Bt-harbouring sample of 21.4% and rate of Bt isolate of 29.4%. Rate of Bt-harbouring sample and rate of Bt isolate in Northwest area were 6.6% and 7.1% respectively. Main serotypes were H4, H5, H19, H10 and H3. In four provinces of Southern China, both rates above were 18.6% and 29.5%, but frequency of Bt distribution was varied seriously in different distinct. Predominant serotypes in soils from Southern China were H3 and H5. Results of bioassay showed that the percentage of strains high active to Heliothis armigera and Plogioidera versicolora were 1.6% and 1.1% in soils from North of China. In contrast to North of China, Bt strains active to H. armigera were 5.3% and none of Bt was effective to P. versicolora in South of China. A strain H27-05 was high toxic to H. armigera and showed temperate toxicity to P. versicolora.
从中国南北1491份土壤样本中分离出221株苏云金芽孢杆菌。已鉴定出所有苏云金芽孢杆菌分离株的H血清型和杀幼虫特性。中国东北和内蒙古地区含苏云金芽孢杆菌土壤样本的比例和苏云金芽孢杆菌分离株的比例分别为12.6%和17.2%。优势血清型为H4、H10、H3、H13、H5和H29。含苏云金芽孢杆菌最丰富的地区是黑龙江省,含苏云金芽孢杆菌样本的比例为21.4%,苏云金芽孢杆菌分离株的比例为29.4%。西北地区含苏云金芽孢杆菌样本的比例和苏云金芽孢杆菌分离株的比例分别为6.6%和7.1%。主要血清型为H4、H5、H19、H10和H3。在中国南方的四个省份,上述两个比例分别为18.6%和29.5%,但苏云金芽孢杆菌在不同地区的分布频率差异很大。中国南方土壤中的优势血清型为H3和H5。生物测定结果表明,中国北方土壤中对棉铃虫和棉小造桥虫高活性菌株的比例分别为1.6%和1.1%。与中国北方相比,中国南方对棉铃虫有活性的苏云金芽孢杆菌菌株为5.3%,且没有苏云金芽孢杆菌对棉小造桥虫有效。菌株H27 - 05对棉铃虫具有高毒性,对棉小造桥虫表现出中等毒性。