[表皮生长因子与角质形成细胞生长因子联合应用对人角膜上皮细胞增殖的影响]
[Effect of combining EGF with KGF on proliferation of human corneal epithelial cells].
作者信息
Gong X, Zhong X
机构信息
Zhongshan Ophthalmic Center, Sum Yat-sen University of Medical Sciences, Guangzhou, China.
出版信息
Yan Ke Xue Bao. 1996 Jun;12(2):107-9.
OBJECTIVE
To select effective drugs for enhancing corneal epithelial wound healing and treating persistent corneal epithelial defect.
METHODS
Epidermal growth factor (EGF) and keratinocyte growth factor (KGF) were added to cultures of human corneal epithelial cells and DNA synthesis in corneal epithelial cells were measured with [3H]-thymidine incorporation and liquid scintillation techniques. The cells were counted in a hemocytometer to determine the doubling time.
RESULTS
10 ng/ml EGF, 10 ng/ml KGF enhanced cellular proliferation effectively (P < 0.01). EGF in combination with KGF, the enhancement was more effective (P < 0.05) and reduced the doubling time.
CONCLUSIONS
Our results suggest that exogenous EGF with KGF can stimulate proliferation of human corneal epithelial cells and it may be of potential use clinically for treatment of persistent corneal epithelial
目的
筛选促进角膜上皮伤口愈合及治疗持续性角膜上皮缺损的有效药物。
方法
将表皮生长因子（EGF）和角质形成细胞生长因子（KGF）添加到人角膜上皮细胞培养物中，采用[3H]-胸腺嘧啶核苷掺入法和液体闪烁技术检测角膜上皮细胞中的DNA合成。用血细胞计数器对细胞进行计数以确定倍增时间。
结果
10 ng/ml EGF、10 ng/ml KGF能有效促进细胞增殖（P < 0.01）。EGF与KGF联合使用时，促进作用更显著（P < 0.05），并缩短了倍增时间。
结论
我们的结果表明，外源性EGF与KGF可刺激人角膜上皮细胞增殖，在临床上可能具有治疗持续性角膜上皮缺损的潜在用途。
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