镰状细胞血红蛋白溶液中的过饱和现象。
Supersaturation in sickle cell hemoglobin solutions.
作者信息
Hofrichter J, Ross P D, Eaton W A
出版信息
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3035-9. doi: 10.1073/pnas.73.9.3035.
The kinetic inhibition of the gelation of hemoglobin S is compared to the change in hemoglobin S soulbility, when the solubility is altered by carbon monoxide, pH, or urea. By means of a new technique, the delay time and the extent of gelation are measured on the same sample. They delay time, td, is found to be proportional to a high power (30-40) of the hemoglobin S solubility. Togehter with the previously reported concentration dependence, this result demonstrates that the rate is proportional to a high power of the supersaturation, S, defined as the ratio of the total hemoglobin S concentration to the equilibrium solubility. The results obey the supersaturation equation td-1 = gammaSn, where gamma is an empirical constant (about 10(-7) sec-1) and n is about 35. The supersaturation equation can successfully account for observations on the kinetics of cell sickling and is therefore used to estimate the increase in the delay time for sickling necessary to produce significant clinical benefit to patients with sickle cell disease.
将血红蛋白S凝胶化的动力学抑制与血红蛋白S溶解度的变化进行比较，当溶解度因一氧化碳、pH值或尿素而改变时。通过一种新技术，在同一样品上测量延迟时间和凝胶化程度。发现延迟时间td与血红蛋白S溶解度的高次方（30 - 40）成正比。连同先前报道的浓度依赖性，该结果表明速率与过饱和度S的高次方成正比，过饱和度S定义为总血红蛋白S浓度与平衡溶解度之比。结果符合过饱和度方程td-1 = γSn，其中γ是一个经验常数（约10(-7)秒-1），n约为35。过饱和度方程能够成功解释关于细胞镰变动力学的观察结果，因此用于估计对镰状细胞病患者产生显著临床益处所需的镰变延迟时间的增加。
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