Gianì S, Qin X, Faoro F, Breviario D
Istituto Biosintesi Vegetali CNR, Milano, Italy.
Planta. 1998 Jul;205(3):334-41. doi: 10.1007/s004250050328.
The effect of the anti-microtubular drug Oryzalin (3,5-dinitro-N4,N4-dipropylsulfanilamide) on growth and elongation of rice (Oryza sativa L. cv. Arborio) roots and coleoptiles was investigated. At 100 nM, Oryzalin strongly reduced primary root elongation, caused loss of cell anisotropy and the disappearance of the cortical microtubule array. Under these conditions the amounts of alpha- and beta-tubulin protein, but not mRNA, were heavily reduced. Similar data were also obtained in coleoptile segments treated with different concentrations of Oryzalin. However, when coleoptile elongation was inhibited by cis-abscisic acid, remarkable decreases in alpha- and beta-tubulin accumulation were observed to occur at the mRNA level but not at the protein level. The transcriptional decreases could be reversed by re-addition of 3-indole acetic acid. Altogether, these data indicate that rice tubulin accumulation can be controlled at different levels, mRNA or protein, in response to Oryzalin or abscisic acid treatments.
研究了抗微管药物氨磺乐灵（3,5-二硝基-N4,N4-二丙基磺胺）对水稻（Oryza sativa L. cv. Arborio）根和胚芽鞘生长及伸长的影响。在100 nM浓度下，氨磺乐灵强烈抑制初生根伸长，导致细胞各向异性丧失以及皮层微管阵列消失。在这些条件下，α-和β-微管蛋白的量大幅减少，但mRNA量未减少。在用不同浓度氨磺乐灵处理的胚芽鞘切段中也获得了类似的数据。然而，当顺式脱落酸抑制胚芽鞘伸长时，在mRNA水平而非蛋白质水平观察到α-和β-微管蛋白积累显著减少。重新添加3-吲哚乙酸可逆转转录水平的下降。总之，这些数据表明，响应氨磺乐灵或脱落酸处理，水稻微管蛋白积累可在mRNA或蛋白质等不同水平受到调控。
