Biotechnology Division, Council of Scientific and Industrial Research-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh, 176061, India.
Mol Biol Rep. 2012 Apr;39(4):3485-90. doi: 10.1007/s11033-011-1121-7. Epub 2011 Jul 3.
The present manuscript describes cloning and expression characterization of alpha-tubulin (CsTUA) gene in an evergreen tree tea [Camellia sinensis (L.) O. Kuntze] in response to winter dormancy (WD), abiotic stresses (sodium chloride, polyethylene glycol, and hydrogen peroxide) and plant growth regulators [abscisic acid (ABA), gibberellic acid (GA(3)), indole-3-butyric acid (IBA), and 6-benzylaminopurine (BA)]. CsTUA encoded a putative protein of 449 amino acids with a calculated molecular weight of 49.6 kDa and an isoelectric point (pI) of 5.09. CsTUA shared 76-84 and 90-95% identity at nucleotide and amino acid level, respectively with TUA genes from other plant species. During the period of active growth (PAG), CsTUA showed maximum expression in floral buds as compared to leaf, stem, fruit and root. Though the transcript was not detectable in the younger leaf tissue during the PAG, the expression was induced within 24 h of the low temperature (LT) treatment. The expression was not modulated by the plant growth regulators either in the tissue harvested during PAG or during WD. It was interesting to record that the expression of CsTUA was up-regulated in response to sodium chloride, polyethylene glycol, and hydrogen peroxide. Data has been discussed on the possible role of CsTUA in imparting tolerance to stresses including to LT so that the tea does not exhibit deciduous nature during winters.
本手稿描述了在常绿茶树[Camellia sinensis（L.）O. Kuntze]中克隆和表达α-微管蛋白（CsTUA）基因，以响应冬季休眠（WD）、非生物胁迫（氯化钠、聚乙二醇和过氧化氢）和植物生长调节剂[脱落酸（ABA）、赤霉素（GA（3））、吲哚-3-丁酸（IBA）和 6-苄基氨基嘌呤（BA）]。CsTUA 编码一个推测的 449 个氨基酸的蛋白质，计算分子量为 49.6 kDa，等电点（pI）为 5.09。CsTUA 在核苷酸和氨基酸水平上与其他植物物种的 TUA 基因分别具有 76-84%和 90-95%的同源性。在活跃生长期（PAG）期间，CsTUA 在花芽中表现出最大的表达，而在叶片、茎、果实和根中则较低。尽管在 PAG 期间较年轻的叶片组织中无法检测到转录物，但在低温（LT）处理后 24 小时内诱导了表达。无论是在 PAG 期间收获的组织中还是在 WD 期间，植物生长调节剂都没有调节其表达。有趣的是，记录到 CsTUA 的表达在响应氯化钠、聚乙二醇和过氧化氢时被上调。已经对 CsTUA 在赋予包括 LT 在内的胁迫耐受方面的可能作用进行了讨论，以便茶树在冬季不会表现出落叶特性。
