Hashimoto Y, Nakamura N, Kuze T, Abe M, Wakasa H
Department of Pathology I, Fukushima Medical College, Japan.
Fukushima J Med Sci. 1997 Dec;43(2):87-98.
Mantle cell lymphoma (MCL) has a characteristic chromosomal translocation, t(11:14) (q13;q32) involving rearrangement of bcl-1 locus, and the key oncogene of bcl-1 locus in PRAD1/cyclin D1 gene that encodes the protein regarding cell cycle. Recently, several studies using immunohistochemical and molecular methods have demonstrated the overexpression of cyclin D1 mRNA/protein in cases of MCL. We have studied immunohistochemical expression of cyclin D1 protein on frozen sections of 27 cases of MCLs and evaluated the relationship between the expression of cyclin D1 and prognosis. Sixteen (59.3%) cases showed intranuclear staining of cyclin D1 protein and 6 of 7 cases examined using RT-PCR methods showed the overexpression of PRAD1/cyclin D1 mRNA. The data indicate that intranuclear staining of cyclin D1 protein is associated with the overexpression of PRAD1/cyclin D1 mRNA. The survival time of cyclin-D1 positive group was shorter than that of cyclin D1-negative group, and there was a significant difference in survival time between the two groups (p < 0.05; log-rank test). These data suggest that the MCLs with overexpression of PRAD1/cyclin D1 protein has poor prognosis, and intranuclear expression of cyclin D1 protein is a useful prognostic marker for MCL.
套细胞淋巴瘤（MCL）具有特征性的染色体易位，即t(11;14)(q13;q32)，涉及bcl-1基因座的重排，以及PRAD1/细胞周期蛋白D1基因中bcl-1基因座的关键致癌基因，该基因编码与细胞周期相关的蛋白质。最近，一些使用免疫组织化学和分子方法的研究表明，在MCL病例中细胞周期蛋白D1 mRNA/蛋白过表达。我们研究了27例MCL冰冻切片上细胞周期蛋白D1蛋白的免疫组织化学表达，并评估了细胞周期蛋白D1表达与预后之间的关系。16例（59.3%）病例显示细胞周期蛋白D1蛋白的核内染色，7例使用逆转录-聚合酶链反应（RT-PCR）方法检测的病例中有6例显示PRAD1/细胞周期蛋白D1 mRNA过表达。数据表明，细胞周期蛋白D1蛋白的核内染色与PRAD1/细胞周期蛋白D1 mRNA的过表达相关。细胞周期蛋白D1阳性组的生存时间短于细胞周期蛋白D1阴性组，两组生存时间存在显著差异（p<0.05；对数秩检验）。这些数据表明，PRAD1/细胞周期蛋白D1蛋白过表达的MCL预后较差，细胞周期蛋白D1蛋白的核内表达是MCL的一个有用的预后标志物。
